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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ngu, M. S. Bhuiyan, M. A. R. Ho, C. Thomson, M. J. Wickneswari, R. |
| Description | Country affiliation: Malaysia Author Affiliation: Ngu MS ( School of Environmental and Natural Resource Sciences, Faculty Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia meesiing@yahoo.com.); Thomson MJ ( Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, Metro Manila, Philippines.); Bhuiyan MA ( School of Environmental and Natural Resource Sciences, Faculty Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia.); Ho C ( Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, Malaysia.); Wickneswari R ( School of Environmental and Natural Resource Sciences, Faculty Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia.) |
| Abstract | Grain weight is a major component of rice grain yield and is controlled by quantitative trait loci. Previously, a rice grain weight quantitative trait locus (qGW6) was detected near marker RM587 on chromosome 6 in a backcross population (BC2F2) derived from a cross between Oryza rufipogon IRGC105491 and O. sativa cv. MR219. Using a BC2F5 population, qGW6 was validated and mapped to a region of 4.8 cM (1.2 Mb) in the interval between RM508 and RM588. Fine mapping using a series of BC4F3 near isogenic lines further narrowed the interval containing qGW6 to 88 kb between markers RM19268 and RM19271.1. According to the Duncan multiple range test, 8 BC4F4 near isogenic lines had significantly higher 100-grain weight (4.8 to 7.5% over MR219) than their recurrent parent, MR219 (P < 0.05). According to the rice genome automated annotation database, there are 20 predicted genes in the 88-kb target region, and 9 of them have known functions. Among the genes with known functions in the target region, in silico gene expression analysis showed that 9 were differentially expressed during the seed development stage(s) from gene expression series GSE6893; however, only 3 of them have known functions. These candidates provide targets for further characterization of qGW6, which will assist in understanding the genetic control of grain weight in rice. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 4 |
| Volume Number | 13 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2014-11-11 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Crosses, Genetic Oryza Sativa Genetics Physical Chromosome Mapping Quantitative Trait Loci Anatomy & Histology Alleles Gene Expression Regulation, Plant Genes, Plant Genetic Association Studies Genotype Organ Size Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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