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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Almeida, C. F. Costa, P. M. A. Barbosa, M. H. P. Bhering, L. L. Baffa, D. C. F. Silveira, G. Soares, B. Peternelli, L. A. |
| Description | Country affiliation: Brazil Author Affiliation: Costa PM ( Laboratório de Biotecnologia e Melhoramento Vegetal, Universidade Federal de Viçosa, Viçosa, MG, Brasil paulomafra@gmail.com.); Almeida CF ( Laboratório de Biotecnologia e Melhoramento Vegetal, Universidade Federal de Viçosa, Viçosa, MG, Brasil.); Silveira G ( Departamento de Fitotecnia, Universidade Federal de Viçosa, Viçosa, MG, Brasil.); Soares B ( Departamento de Fitotecnia, Universidade Federal de Viçosa, Viçosa, MG, Brasil.); Baffa DC ( Departamento de Fitotecnia, Universidade Federal de Viçosa, Viçosa, MG, Brasil.); Peternelli LA ( Departamento de Estatística, Universidade Federal de Viçosa, Viçosa, MG, Brasil.); Bhering LL ( Departamento de Genética, Universidade Federal de Viçosa, Viçosa, MG, Brasil.); Barbosa MH ( Departamento de Fitotecnia, Universidade Federal de Viçosa, Viçosa, MG, Brasil.) |
| Abstract | Superior inbred clones selected in S1 families can integrate an individual reciprocal recurrent selection program in sugarcane by eliminating the genetic load of the population and exploring superior hybrid combinations. Molecular markers can be used for reliable identification of the true selfing-derived clones in these S1 populations. The objective of this study was to confirm true self-fertilized individuals in sugarcane families using microsatellite markers aimed at the use of self-fertilized plants in an individual reciprocal recurrent selection strategy. Self-fertilized individuals from five cultivars were genotyped with eight simple sequence repeat (SSR) markers. The markers generated 62 polymorphic markers, with an average of seven polymorphic alleles across the cultivars tested. Three loci revealed highly informative bands and were used to assess the level of selfing in five S1 families. Selfing in these families ranged from 71.7 to 97.6%. The SSR loci provide a reliable and accurate method to identify S1 progenies in sugarcane crosses and can be used as a tool to assist selection strategies in sugarcane breeding programs. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 4 |
| Volume Number | 13 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2014-10-31 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Microsatellite Repeats Genetics Saccharum Selection, Genetic Self-fertilization Breeding Crosses, Genetic Dna, Plant Genotype Pollination Polymerase Chain Reaction Polymorphism, Genetic Reproducibility Of Results Classification Species Specificity Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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