| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Ding, Y. Y. Zhang, X. D. Huang, L. Zhang, S. J. Yin, Z. J. Liu, L. Y. Zhou, J. |
| Description |
Country affiliation: China Author Affiliation: Ding YY ( Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui, China.); Liu LY ( Department of Veterinary, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui, China.); Zhou J ( Department of Veterinary, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui, China.); Zhang XD ( Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui, China.); Huang L ( Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui, China.); Zhang SJ ( Department of Veterinary, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui, China.); Yin ZJ ( Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui, China yinzongjun@ahau.edu.cn.) |
| Abstract |
The aim of this study was to determine the imprinting status of the Ras protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1) gene in domestic pigs. In this study, a 228-bp partial sequence located in exon 14 and a 193-bp partial sequence located in exon 1 of the Rasgrf1 gene in domestic pigs were obtained. A novel single nucleotide polymorphism, a G/A transition, was identified in Rasgrf1 exon 14, and then the reciprocal Berkshire x Wannan black F1 hybrid model and the reverse transcription-polymerase chain reaction-restriction fragment length polymorphism method were used to detect the imprinting status of the porcine Rasgrf1 gene at the 1-day-old developmental stage. Imprinting analysis showed that, compared to the imprinted expression of the Rasgrf1 gene in mouse and rat, a variable imprinting status was observed in domestic pigs. In principle, the porcine Rasgrf1 gene was maternally expressed in the liver and small intestine, paternally expressed in the lung, and biallelically expressed in brain, heart, spleen, kidney, stomach, pancreas, fat, testis, ovary, longissimus dorsi, and pituitary tissues. In conclusion, our results indicated that the Rasgrf1 gene shows both species- and tissue-specific variation in imprinted expression. |
| e-ISSN |
16765680 |
| Journal |
Genetics and Molecular Research |
| Issue Number |
4 |
| Volume Number |
13 |
| Language |
English |
| Publisher |
Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date |
2014-12-12 |
| Publisher Place |
Brazil |
| Access Restriction |
Open |
| Subject Keyword |
Genomic Imprinting Polymorphism, Single Nucleotide Genetics Sus Scrofa Ras-grf1 Animals Exons Gene Expression Regulation, Developmental Guanine Nucleotides Mice Growth & Development Tissue Distribution Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type |
Text |
| Resource Type |
Article |
