| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Francisco, M. R. Silveira, L. F. Azeredo, R. M. A. Simpson, J. G. P. Laganaro, N. M. Camargo, C. Oliveira, P. R. R. Costa, M. C. Davanço, P. V. |
| Description |
Country affiliation: Brazil Author Affiliation: Costa MC ( Departamento de Ciências Ambientais, Universidade Federal de São Carlos, Campus de Sorocaba, Sorocaba, SP, Brasil.); Camargo C ( Departamento de Ciências Ambientais, Universidade Federal de São Carlos, Campus de Sorocaba, Sorocaba, SP, Brasil.); Laganaro NM ( Departamento de Ciências Ambientais, Universidade Federal de São Carlos, Campus de Sorocaba, Sorocaba, SP, Brasil.); Oliveira PR ( Departamento de Ciências Ambientais, Universidade Federal de São Carlos, Campus de Sorocaba, Sorocaba, SP, Brasil.); Davanço PV ( Departamento de Ciências Ambientais, Universidade Federal de São Carlos, Campus de Sorocaba, Sorocaba, SP, Brasil.); Azeredo RM ( Departamento de Ciências Ambientais, Universidade Federal de São Carlos, Campus de Sorocaba, Sorocaba, SP, Brasil.); Simpson JG ( CRAX, Sociedade de Pesquisa do Manejo e da Reprodução da Fauna Silvestre, Contagem, MG, Brasil.); Silveira LF ( Seção de Aves, Museu de Zoologia da Universidade de São Paulo, São Paulo, SP, Brasil.); Francisco MR ( Departamento de Ciências Ambientais, Universidade Federal de São Carlos, Campus de Sorocaba, Sorocaba, SP, Brasil mercival@ufscar.br.) |
| Abstract |
Cracids are medium to large frugivorous birds that are endemic to the Neotropics. Because of deforestation and overhunting, many species are threatened. The conservation of several species has relied on captive breeding and reintroduction in the wild, but captive populations may be inbred. Microsatellite tools can permit the construction of genetic pedigrees to reduce inbreeding, but only a few loci are available for this group of birds. Here, we present 10 novel polymorphic microsatellite loci and the cross-amplification of these and of 10 additional loci available in the literature in a panel of 5 cracid species, including 3 species with high conservation concern. We provide the first polymorphic loci for the jacutinga, Aburria jacutinga (N = 8), and red-billed curassow, Crax blumenbachii (N = 9), and additional loci for bare-faced curassow, C. fasciolata (N = 8), Alagoas curassow, Pauxi mitu (N = 5), and razor-billed curassow, P. tuberosa (N = 5). The average number of alleles was 2.9 for A. jacutinga, 2.7 for C. blumenbachii, 3.5 for C. fasciolata, 2.6 for P. mitu, and 5.7 for P. tuberosa. The mean expected heterozygosities were 0.42, 0.40, 0.48, 0.37, and 0.59, respectively. The average probabilities that the set of loci would not exclude a pair of parents of an arbitrary offspring were 2.9% in A. jacutinga, 1% in C. blumenbachii, 0.5% in C. fasciolata, 0.4% in P. mitu, and 0.002% in P. tuberosa suggesting that these loci may be adequate for parentage analysis and to implement ex situ genetic management plans. |
| e-ISSN |
16765680 |
| Journal |
Genetics and Molecular Research |
| Issue Number |
4 |
| Volume Number |
13 |
| Language |
English |
| Publisher |
Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date |
2014-11-27 |
| Publisher Place |
Brazil |
| Access Restriction |
Open |
| Subject Keyword |
Conservation Of Natural Resources Galliformes Genetics Microsatellite Repeats Alleles Animals Genetic Loci Heterozygote Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type |
Text |
| Resource Type |
Article |
