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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Li, H. J. Liu, D. Zhao, Z. G. Jiao, Z. L. |
| Description | Country affiliation: China Author Affiliation: Liu D ( Department of Dermatology, PLA General Hospital, Beijing, China.); Zhao ZG ( Department of Dermatology, PLA General Hospital, Beijing, China.); Jiao ZL ( Department of Dermatology, Clinic of Navy Headquarters, Beijing, China.); Li HJ ( Department of Dermatology, PLA General Hospital, Beijing, China HengjinLi@hotmail.com.) |
| Abstract | Melanoma is a malignant tumor and one of the most frequent metastatic cancers. This study was conducted to identify differential expression genes (DEGs) and single nucleotide variations (SNVs) in metastatic melanoma. We analyzed microarray data of GSE23056 downloaded from the Gene Expression Omnibus, including two normal samples (skinN1 and skinN2) and 2 metastatic melanoma samples (skinT and lymphT). We not only compared DEGs in metastatic melanoma samples with normal samples (lymphT_skinN and skinT_skinN), but also compared DEGs between two metastatic melanoma types (lymphT_skinT). SNVs were identified by using Burrows-Wheeler Aligner and Cufflinks in metastatic melanoma samples using RNA-seq. Sequence Alignment/Map tools and the ANNOVAR software were used to analyze and annotate SNVs. We identified 18 significantly common DEGs in lymphT_skinN and skinT_skinN and one common gene, YBX1, in lymphT_skinN, skinT_skinN, and lymphT_skinT. We identified 49,534, 48,118, 63,812, and 33,096 SNVs in skinN1, skinN2, skinT, and lymphT, respectively. Twenty-nine SNVs were located in exonic regions of two DEGs, HLA-B and TSPAN10. SNVs that exist only in tumors were located in MARVELD1, SLC16A3, and VAV3. The DEGs screened in our study are potential biomarkers for metastatic melanoma therapy. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 4 |
| Volume Number | 13 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2014-10-07 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Gene Expression Profiling Gene Expression Regulation, Neoplastic Melanoma Genetics Pathology Polymorphism, Single Nucleotide Chromosomes, Human Genome-wide Association Study Neoplasm Metastasis Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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