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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | An, C. M. Hong, C. G. Myeong, J. I. Park, J. Y. Yang, S. G. Moon, T. S. Hwang, H. K. An, H. S. |
| Spatial Coverage | Republic of Korea |
| Description | Author Affiliation: An HS ( Biotechnology Research Division, National Fisheries Research and Development Institute, Busan, Korea hsan97@korea.kr.); Yang SG ( Future Aquaculture Research Center, Jeju-do, Korea.); Moon TS ( Future Aquaculture Research Center, Jeju-do, Korea.); Park JY ( Aquaculture Industry Division, National Fisheries Research and Development Institute, Yeosu, Korea.); Hong CG ( Aquaculture Industry Division, National Fisheries Research and Development Institute, Yeosu, Korea.); Hwang HK ( Aquaculture Management Division, National Fisheries Research and Development Institute, Busan, Korea.); Myeong JI ( Aquaculture Management Division, National Fisheries Research and Development Institute, Busan, Korea.); An CM ( Biotechnology Research Division, National Fisheries Research and Development Institute, Busan, Korea.) |
| Abstract | The kelp grouper Epinephelus bruneus (Perciformes: Haemulidae), is one of the most economically important fishery resources in Korea. This fish is regarded as a target for prospective aquaculture diversification; therefore, maintenance of stock quality is important. To investigate the effects of current artificial reproduction in a hatchery facility, genetic variation in wild-caught broodstock and hatchery-produced offspring of kelp grouper was analyzed using eight polymorphic nuclear microsatellite DNA loci; 77 alleles were identified. Allelic variability ranged from 2 to 22 in the broodstock and from 1 to 10 in the offspring. The average observed and expected heterozygosities were 0.620 and 0.623 in the broodstock and 0.600 and 0.513 in the offspring, respectively. The possibility of a recent genetic bottleneck was suggested in both populations of E. bruneus. The minor, but significant, genetic differentiation (FST = 0.047, P < 0.05) observed was mainly due to statistically significant reductions in the number of alleles in the offspring compared with the broodstock, suggesting that these genetic changes could be related to genetic drift. Our results demonstrate the usefulness of microsatellite markers to monitor genetic variation and raise concerns about potential harmful genetic effects of inappropriate hatchery procedures. Therefore, genetic variation between broodstock and offspring in a hatchery should be monitored in both breeding and release programs as a routine hatchery operation, and inbreeding should ideally be controlled to improve kelp grouper hatchery management. Our data provide a useful genetic basis for future planning of sustainable culture and management of E. bruneus in fisheries. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 4 |
| Volume Number | 13 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2014-11-14 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Animals, Wild Genetics Genetic Variation Microsatellite Repeats Perciformes Alleles Animals Breeding Gene Frequency Genetic Drift Genetic Loci Heterozygote Kelp Comparative Study Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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