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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhang, J. Ma, W. Y. |
| Description | Country affiliation: China Author Affiliation: Zhang J ( Medical College, Shandong University, Jinan, Shandong, China.); Ma WY ( Department of Dermatology, Qilu Hospital of Shandong University, Jinan, China JinZHANG6@yeah.net.) |
| Abstract | Decades of research have provided the data to confirm the hypothesis that there is bidirectional communication between the central nervous system and the immune system in psoriasis pathogenesis, but the contribution of the cutaneous neural system remains underexplored. In this study, we evaluated the molecular mechanisms by which nerve growth factor (NGF) regulates hypoxia-inducible factor-1 (HIF-1 ) and vascular endothelial growth factor (VEGF) production. The mRNA and protein levels of VEGF secretion from HaCaT cells by NGF were increased in a concentration-dependent manner. In addition, the NGF- induced increase in VEGF is accompanied by an increase in HIF-1 , but not HIF-2 or HIF-1ß. However, this increase is abrogated by pretreatment with a mammalian target of rapamycin (mTOR) inhibitor rapamycin. Pharmacologic inhibitors of the Trk tyrosine kinase, PI-3 kinase, and mTOR pathways prevent NGF-stimulated increases in HIF-1 and VEGF. Mutation of the siRNA-mediated silencing of HIF-1 expression blocks NGF-induced increases in VEGF transcription. Our study indicates that NGF regulates the expression of VEGF through the PI3K/mTOR signaling pathway in human HaCaT keratinocytes. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 4 |
| Volume Number | 13 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2014-01-24 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Keratinocytes Metabolism Nerve Growth Factor Pharmacology Phosphatidylinositol 3-kinases Signal Transduction Drug Effects Tor Serine-threonine Kinases Vascular Endothelial Growth Factor A Genetics Cell Line Flavonoids Gene Knockdown Techniques Hypoxia-inducible Factor 1, Alpha Subunit Mitogen-activated Protein Kinases Rna, Messenger Rna, Small Interfering Sirolimus Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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