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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhao, D. X. Chen, L. Q. Wu, P. Li, E. C. Qin, J. G. Zhang, H. Qin, C. J. |
| Description | Country affiliation: China Author Affiliation: Zhao DX ( Department of Biology, East China Normal University, Shanghai, China.); Chen LQ ( Department of Biology, East China Normal University, Shanghai, China lqchen@bio.ecnu.edu.cn.); Qin JG ( School of Biological Sciences, Flinders University, Adelaide, Australia.); Qin CJ ( Department of Biology, East China Normal University, Shanghai, China.); Zhang H ( Department of Biology, East China Normal University, Shanghai, China.); Wu P ( Department of Biology, East China Normal University, Shanghai, China.); Li EC ( Department of Biology, East China Normal University, Shanghai, China.) |
| Abstract | A cytosolic manganese superoxide dismutase gene (Es-cMnSOD) was cloned from the Chinese mitten crab Eriocheir sinensis, using reverse transcription-polymerase chain reaction and the rapid amplification of cDNA ends. The open reading frame of Es-cMnSOD is 867 bp in length and encodes a 288-amino acid protein without a signal peptide. The calculated molecular mass of the translated protein of Es-cMnSOD is 31.43 kDa, with an estimated isoelectric point of 6.30. The deduced amino acid sequence of Es-cMnSOD has similarities of 90, 89, 84, 87, and 81% to those of white shrimp Litopenaeus vannamei MnSOD, black tiger shrimp Penaeus monodon MnSOD, giant freshwater prawn Macrobrachium rosenbergii MnSOD, blue crab Callinectes sapidus MnSOD, and red swamp crayfish Procambarus clarkii MnSOD, respectively. Es-cMnSOD contains a manganese superoxide dismutase domain (DVWEHAYY) and 4 conserved amino acids responsible for binding manganese. Es-cMnSOD was expressed in the hemocytes, eyestalk, muscle, intestine, gill, and hepatopancreas. Es-cMnSOD transcripts in hemocytes of E. sinensis increased at 1.5 and 48 h after injection of Aeromonas hydrophila, indicating that the induction of the SOD system response occurred within a short period of time. This study suggests that MnSOD may play a critical role in crab immunity, allowing efficient activation of an early innate immune response in the crab. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 4 |
| Volume Number | 13 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2014-11-11 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Brachyura Enzymology Cytosol Superoxide Dismutase Genetics Amino Acid Motifs Amino Acid Sequence Animals Dna, Complementary Gene Expression Regulation, Enzymologic Molecular Sequence Data Phylogeny Rna, Messenger Metabolism Sequence Alignment Sequence Homology, Amino Acid Chemistry Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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