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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Li, C. M. Wu, J. Wang, G. L. Bai, J. Y. Huang, S. Li, L. |
| Spatial Coverage | China |
| Description | Country affiliation: China Author Affiliation: Wu J ( College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.); Bai JY ( College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.); Li L ( College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.); Huang S ( College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.); Li CM ( College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.); Wang GL ( College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China glwang@njau.edu.cn.) |
| Abstract | DNA sequencing, nested polymerase chain reaction (PCR), and PCR-restriction fragment length polymorphism were used to investigate mutations in the coding regions of the bovine myeloid antimicrobial peptide-28 (BMAP-28) and mannan-binding lectin-associated serine protease-2 (MASP-2) genes in 249 Chinese Holstein dairy cows. The associations between the polymorphisms and somatic cell score (SCS) were analyzed to determine the potential of these genes as genetic markers for breeding mastitis-resistant dairy cattle. The results revealed a C-86G synonymous mutation in the BMAP-28 gene that caused no alteration in the amino acid sequence. G553A mutation was found in the MASP-2 gene that led to the substitution of glycine with serine. The chi-square test showed that the G553A mutation was in Hardy-Weinberg equilibrium in the Chinese Holstein dairy cows, whereas the C-86G mutation was not. The variance analysis of the influence of group loci and different genotypes on the SCS showed that the SCS of genotype CC was significantly lower than those of genotypes CG and GG (P < 0.05) of the C-86G mutation, and that the SCS of genotype GG was significantly lower than those of genotypes GA and AA (P < 0.05) of the G553A mutation. Genotype combination analysis showed that the combination of the BMAP-28 genotype CC and the MASP-2 genotype GG was the best one, in which the SCS was significantly lower than those in the other combinations. Thus, this combination might be useful as a molecular and genetic marker of mastitis in Chinese Holstein dairy cows. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 1 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-01-15 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Genetics Mannose-binding Protein-associated Serine Proteases Polymorphism, Single Nucleotide Proteins Alleles Animals Gene Frequency Genetic Association Studies Genetic Loci Heterozygote Least-squares Analysis Molecular Sequence Data Mutation Quantitative Trait, Heritable Sequence Analysis, Dna Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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