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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Shao, C. Lei, Y. X. Lian, Y. Y. Lu, Q. He, C. C. Lei, Z. N. |
| Description | Country affiliation: China Author Affiliation: Lei YX ( School of Public Health, Guangzhou Medical University, Guangzhou, China yixionglei@yeah.net.); Lu Q ( School of Public Health, Guangzhou Medical University, Guangzhou, China.); Shao C ( School of Public Health, Guangzhou Medical University, Guangzhou, China.); He CC ( School of Public Health, Guangzhou Medical University, Guangzhou, China.); Lei ZN ( School of Public Health, Guangzhou Medical University, Guangzhou, China.); Lian YY ( School of Public Health, Guangzhou Medical University, Guangzhou, China.) |
| Abstract | We aimed to evaluate the toxicity of long-term exposure to different cadmium (Cd) doses in rats and expression profiles of DNA repair-related genes. The model rats were exposed to different concentrations of CdCl2 for 3 months, and 5 DNA repair-related genes - hMSH2, MLH1, XRCC1, hOGG1, ERCC1 - were cloned in different tissues, including the liver, kidney, heart, and lung. Accumulated amounts of Cd were detected in the tissues. Gene and protein detections were conducted via fluorescence quantitative real-time polymerase chain reaction and Western blotting, respectively. Methylated sequences of the 5 DNA repair-related gene promoters were used to investigate whether the low expression levels of the genes were related to methylation of the promoter. In the Cd-exposed group, 3 DNA repair genes (i.e., XRCC1, hOGG1, and ERCC1) significantly decreased in the rat liver, kidney, heart, and lung according to the ß-actin internal standard (P < 0.01). Western blotting indicated the same trend for the different tissues. Each of the DNA repair genes had special characteristics; for example, hOGG1 gene expression decreased by 75% in the kidney, and XRCC1 gene expression decreased by 5% in the liver and heart when compared to the control group (P < 0.01). A negative correlation between the DNA repair gene expression levels and the cumulative levels of Cd was also suggested by malignancy pathology. The expression levels of 3 DNA repair genes (i.e., ERCC1, XRCC1, and hOGG1) played an important role in the rat response to Cd exposure but not DNA methylated protection. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 1 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-01-26 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Cadmium Toxicity Dna Repair Genetics Gene Expression Profiling Gene Expression Regulation Drug Effects Organ Specificity Animals Blotting, Western Dna Methylation Rats, Sprague-dawley Real-time Polymerase Chain Reaction Sequence Analysis, Dna Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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