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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Atak, Ç. Çelik, Ö. |
| Description | Country affiliation: Turkey Author Affiliation: Çelik Ö ( Department of Molecular Biology and Genetics, Faculty of Science and Letters, Istanbul Kultur University, Ataköy, Istanbul, Turkey ocelik@iku.edu.tr.); Atak Ç ( Department of Molecular Biology and Genetics, Faculty of Science and Letters, Istanbul Kultur University, Ataköy, Istanbul, Turkey.) |
| Abstract | Salinity is one of the major problems limiting the yield of agricultural products. Radiation mutagenesis is used to improve salt-tolerant mutant plants. In this study, we aimed to improve salt-tolerant mutants of two oriental tobacco varieties. One thousand seeds of each variety (M0) were irradiated with 100, 200, 300, and 400 Gy gamma rays by Cs-137 gamma. In the M1 generation, 2999 single plants were harvested. The next season, these seeds were bulked and planted to obtain M2 progeny. The seeds of 1900 M2 plants were picked separately. Salinity tolerance was tested in the M3 generation. Among M3 plantlets, 10 salt-tolerant tobacco mutants were selected. According to the results of the selection studies, 100- and 200-Gy gamma radiation doses were the effective doses to obtain the desired mutants. Glutathione reductase enzyme activities of salt-tolerant tobacco mutants were determined biochemically as a stress-tolerance marker. The differences between control and salt-tolerant mutants belonging to the Akhisar 97 and Izmir Özbas tobacco varieties were evaluated by random amplified polymorphic DNA analysis. The total polymorphism rate was 73.91%. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 1 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-02-13 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Mutation Random Amplified Polymorphic Dna Technique Salt-tolerant Plants Genetics Tobacco Radiation Effects Dna Primers Dna, Plant Dose-response Relationship, Radiation Gamma Rays Germination Glutathione Reductase Metabolism Mutagenesis Polymorphism, Genetic Salinity Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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