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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhao, C. G. Wu, Y. B. Zhou, P. |
| Description | Country affiliation: China Author Affiliation: Zhao CG ( Department of Pediatric Nephrology and Rheumatology, Shengjing Hospital of China Medical University, Shenyang, China.); Zhou P ( Department of Pediatric Internal Medicine, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.); Wu YB ( Department of Pediatric Nephrology and Rheumatology, Shengjing Hospital of China Medical University, Shenyang, China gczpcn@163.com.) |
| Abstract | We investigated the impact and signal transduction mechanisms of epigallocatechin-3-gallate (EGCG) on transdiffer-entiation of renal tubular epithelial cells. Rat renal tubular epithelial cells (NRK-52E) were randomly divided into a normal control group, transforming growth factor (TGF)-b1-induced group (10 ng/mL), and intervention groups with 200 mg/L EGCG + 10 ng/mL TGF-b1 and 400 mg/L EGCG + 10 ng/mL TGF-b1. Tested cells were collected after 48 h. Levels of a-smooth muscle actin ( -SMA) and cytokeratin-18 were detected using immunohistochemical methods. Western blotting was used to detect cytoplasmic Pi-extracellular receptor kinase 1/2 (ERK1/2), Pi-Smad3 protein, and nuclear b-catenin protein. mRNA expression of ERK2, Smad3, and ß-catenin was measured by reverse transcription-polymerase chain reaction. After induction by TGF-b1, cytokeratin-18 expression in the renal tubular epithelial cells decreased and a-SMA expression appeared. mRNA expression of cytoplasmic Pi-Smad3 and Pi-ERK1/2, Smad3, ERK2, and b-catenin protein expression increased, while ß-catenin mRNA decreased. These changes were reduced after intervention by EGCG. EGCG may be helpful for maintaining the renal tubular epithelial cell phenotype and reducing the degree of TGF-b1- induced cell transdifferentiation, which may be related to the signal transduction pathway of ERK, Smad3, and ß-catenin. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 1 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-03-30 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Catechin Analogs & Derivatives Epithelial Cells Drug Effects Epithelial-mesenchymal Transition Kidney Tubules Protective Agents Pharmacology Signal Transduction Animals Cell Line Extracellular Signal-regulated Map Kinases Genetics Metabolism Gene Expression Smad3 Protein Beta Catenin Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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