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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Strömberg, Mattias Bejhed, Rebecca S. Svedlindh, Peter Tian, Bo |
| Description | Country affiliation: Sweden Author Affiliation: Tian B ( Department of Engineering Sciences, Uppsala University, Box 534, SE-751 21 Uppsala, Sweden.); Bejhed RS ( Department of Engineering Sciences, Uppsala University, Box 534, SE-751 21 Uppsala, Sweden.); Svedlindh P ( Department of Engineering Sciences, Uppsala University, Box 534, SE-751 21 Uppsala, Sweden.); Strömberg M ( Department of Engineering Sciences, Uppsala University, Box 534, SE-751 21 Uppsala, Sweden. Electronic address: mattias.stromberg@angstrom.uu.se.) |
| Abstract | A turn-on competitive immunoassay using a low-cost Blu-ray optomagnetic setup and two differently sized magnetic particles (micron-sized particles acting as capture particles and nano-sized particles acting as detection particles) is here presented. For Salmonella detection, a limit of detection of 8×10(4)CFU/mL is achieved within a total assay time of 3h. The combination of a competitive strategy and an optomagnetic setup not only enables a turn-on read-out format, but also results in a sensitivity limit about a factor of 20 times lower than of volumetric magnetic stray field detection device based immunoassays. The improvement of sensitivity is enabled by the formation of immuno-magnetic aggregates providing steric hindrance protecting the interior binding sites from interaction with the magnetic nanoparticle labels. The formation of immuno-magnetic aggregates is confirmed by fluorescence microscopy. The system exhibits no visible cross-reaction with other common pathogenic bacteria, even at concentrations as high as 10(7)CFU/mL. Furthermore, we present results when using the setup for a qualitative and homogeneous biplex immunoassay of Escherichia coli and Salmonella typhimurium. |
| ISSN | 09565663 |
| Volume Number | 77 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-03-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Cd-rom Immunoassay Immunomagnetic Separation Salmonella Immunology Isolation & Purification Equipment Design Equipment Failure Analysis Magnetics Optical Devices Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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