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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Gui, Guo-Feng Chai, Ya-Qin Zhuo, Ying Xiang, Yun Yuan, Ruo |
| Description | Country affiliation: Switzerland Author Affiliation: Gui GF ( Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China); Zhuo Y ( Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: yingzhuo@swu.edu.cn.); Chai YQ ( Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.); Xiang Y ( Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.); Yuan R ( Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: yuanruo@swu.edu.cn.) |
| Abstract | In this work, a novel Ru complex and hollow gold nanoparticles branched-poly(N-(3-aminopropyl)methacrylamide) hydrogel composites (pNAMA-Ru-HGNPs) were prepared and used as electrogenerated chemiluminescence (ECL) signal probe to construct aptasensor for ultrasensitive detection of thrombin (TB). Herein, [Ru(phen)2(cpaphen)](2+) linked N-(3-aminopropyl)methacrylamide and hollow gold nanoparticles functionalized N-(3-aminopropyl)methacrylamide were used as two polymer monomers to prepare pNAMA-Ru-HGNPs composites via free-radical polymerization. The obtained hydrogel composite, containing amount of Ru complex and HGNPs, were used as effective tag-carriers for the immobilization of thrombin binding aptamer II (TBA II) to form the pNAMA-Ru-HGNPs labeled TBA II (pNAMA-Ru-HGNPs-TBA II). For building the interface of the aptasensor, dendritic gold nanoparticles reduced by poly(ethyleneimine) (PEI@DGNPs) were modified on the carbon nanotube-nafion (CNTs-Nf) coated electrode through electrostatic adsorption, which was used not only as matrix for immobilization of thrombin binding aptamer I (TBA I) but also as enhancer to amplify the ECL signal because PEI is an efficient co-reactant of Ru complex. Target TB was sandwiched between pNAMA-Ru-HGNPs-TBA II and TBA I, resulting in the ECL signals relevant to the TB concentrations. Combining the novel pNAMA-Ru-HGNPs containing amount of Ru complex as the ECL signal probe and PEI@DGNPs as the enhancer for signal amplification, the sandwich ECL aptasensor was constructed for the detection of TB with a wide range of 1.0 fM to 10 pM and a low detection of 0.54 fM. |
| ISSN | 09565663 |
| Volume Number | 77 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-03-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Aptamers, Nucleotide Chemistry Conductometry Instrumentation Luminescent Measurements Metal Nanoparticles Ruthenium Thrombin Analysis Equipment Design Equipment Failure Analysis Gold Hydrogels Ultrastructure Molecular Probe Techniques Molecular Probes Chemical Synthesis Nanopores Porosity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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