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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Park, Byung Sun Thai, Hien Bao Dieu Ahn, Dae-Ro Min, Sun-Joon Yu, Jin Kyung Park, Yeon-Joon |
| Description | Author Affiliation: Thai HB ( Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea); Yu JK ( Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Banpo-daero 222 Seocho-gu, Seoul 137-701, Republic of Korea.); Park BS ( Center for Neuro-Medicine, Korea Institute of Science and Technology, Republic of Korea.); Park YJ ( Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Banpo-daero 222 Seocho-gu, Seoul 137-701, Republic of Korea. Electronic address: yjpk@catholic.ac.kr.); Min SJ ( Department of Applied Chemistry, Hanyang University, Ansan, Gyeonggi-do 15588, Republic of Korea. Electronic address: sjmin@hanyang.ac.kr.); Ahn DR ( Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea) |
| Abstract | We devised and synthesized a fluorogenic substrate of ß-lactamases as a probe to detect the activity of the enzymes. Fluorescence of the probe emitted upon treatment of a ß-lactamase and increased proportionally to the concentration of the enzyme, demonstrating its sensing property for the activity of the enzyme. We also showed that the probe could be utilized to assay the enzyme and to determine kinetic parameters of the enzyme. Moreover, the probe was able to detect resistance to the third-generation oxyimino-cephalosporin-derived antibiotics such as cefotaxime and ceftazidime. In particular, the probe could identify the ceftazidime-resistance in bacteria that was not detectable using conventional pH-sensing materials, indicating the practical utility of the probe. |
| ISSN | 09565663 |
| Volume Number | 77 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-03-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Bacteria Enzymology Isolation & Purification Drug Resistance, Bacterial Drug Effects Fluorescent Dyes Chemistry Spectrometry, Fluorescence Instrumentation Beta-lactamases Analysis Biosensing Techniques Equipment Design Equipment Failure Analysis Molecular Probe Techniques Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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