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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Azimzadeh, Mostafa Naderi-Manesh, Hossein Rahaie, Mahdi Nasirizadeh, Navid Ashtari, Khadijeh |
| Description | Country affiliation: Iran Author Affiliation: Azimzadeh M ( Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, PO Box: 14395-1561, Tehran, Iran.); Rahaie M ( Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, PO Box: 14395-1561, Tehran, Iran. Electronic address: mrahaie@ut.ac.ir.); Nasirizadeh N ( Department of Textile and Polymer Engineering, Yazd Branch, Islamic Azad University, PO Box: 89195-155, Yazd, Iran. Electronic address: nasirizadeh@iauyazd.ac.ir.); Ashtari K ( Department of Nanobiotechnology/Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, PO Box: 14115-154, Tehran, Iran.); Naderi-Manesh H ( Department of Nanobiotechnology/Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, PO Box: 14115-154, Tehran, Iran. Electronic address: naderman@modares.ac.ir.) |
| Abstract | Circulating miRNAs are emerging as novel reliable biomarkers for early detection of cancer diseases. Through combining the advantages of electrochemical methods and nanomaterials with the selectivity of the oligo-hybridization-based biosensors, a novel electrochemical nanobiosensor for plasma miR-155 detection have demonstrated here, based on thiolated probe-functionalized gold nanorods (GNRs) decorated on the graphene oxide (GO) sheet on the surface of the glassy carbon electrode (GCE). The reduction signals of a novel intercalating label Oracet Blue (OB), were measured by differential pulse voltammetry (DPV) method. The transmission electron microscope (TEM) imaging, UV-vis spectrophotometry, cyclic voltammetry (CV), field emission scanning electron microscope (FE-SEM) imaging and energy dispersive spectroscopy (EDS) were proved the right synthesis of the GNRs and correct assembly of the modified electrode. The electrochemical signal had a linear relationship with the concentration of the target miRNA ranging from 2.0 fM to 8.0 pM, and the detection limit was 0.6 fM. Furthermore, the nanobiosensor showed high Specificity, and was able to discriminate sharply between complementary target miRNA, single-, three-base mismatch, and non-complementary miRNA. Alongside the outstanding sensitivity and selectivity, this nanobiosensor had great storage ability, reproducibility, and showed a decent response in the real sample analysis with plasma. In conclusion, the proposed electrochemical nanobiosensor could clinically be used in the early detection of the breast cancer, by direct detection of the plasma miR-155 in real clinical samples, without a need for sample preparation, RNA extraction and/or amplification. |
| ISSN | 09565663 |
| Volume Number | 77 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-03-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Breast Neoplasms Diagnosis Conductometry Early Detection Of Cancer Micrornas Blood Nanotubes Chemistry Tumor Markers, Biological Equipment Design Equipment Failure Analysis Gold Graphite Metal Nanoparticles Ultrastructure Nanotechnology Oxides Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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