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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Yang, Juan Shen, Huawei Xiang, Hua Tao, Yiyi Zhang, Xing Xie, Guoming |
| Description | Author Affiliation: Yang J ( Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China.); Shen H ( Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China.); Zhang X ( Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China.); Tao Y ( Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China.); Xiang H ( Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China. Electronic address: xianghuacq@163.com.); Xie G ( Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China. Electronic address: guomingxie@cqmu.edu.cn.) |
| Abstract | Gold nanoparticles composite graphitized mesoporous carbon nanoparticles (GMCs@AuNPs) biocomposite with the signal amplification capability was successfully synthesized for use in an immunoassay for penicillin binding protein 2 a (PbP2a). The polyamidoamine (PAMAM) dendrimers were first electrodeposited onto the Au electrode can greatly increase the amount of the captured antibodies. Protein A was used to properly orientate immobilized antibody against PbP2a, which strongly improved specificity of the antigen-antibody binding. Hollow gold nanospheres (HGNPs) as effective nanocarriers have been synthesized by sacrificial galvanic replacement of cobalt nanoparticles capable of encapsulating doxorubicin (Dox). The obtained HGNPs@Dox bionanocomposite was used for further loading of detection antibody (Ab2) to form the HGNPs@Dox@Ab2 bioconjugate. Then, the differential pulse voltammetric signals related to the concentration of PbP2a for Dox could be detected, and the immunosensor exhibited a detection limit as low as 0.65 pg mL(-1) (at an S/N ratio of 3). The proposed method with an excellent differentiation ability showed high sensitivity and specificity. The morphologies and electrochemistry properties of the composites were investigated by scanning electron microscopy, electrochemical characterization, UV-visible absorption spectroscopy, fluorescence spectrophotometer and Malvern laser particle size analyzer, respectively. In addition, the basic approach described here would be applicable towards developing biodetection assays against other important targets. Moreover, the bioconjugate of HGNPs@Dox is also a promising pattern to delivery Dox in vivo for anticancer therapy. |
| ISSN | 09565663 |
| Volume Number | 77 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-03-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Bacterial Proteins Analysis Conductometry Instrumentation Doxorubicin Chemistry Graphite Immunoassay Metal Nanoparticles Penicillin-binding Proteins Adsorption Antineoplastic Agents Carbon Equipment Design Equipment Failure Analysis Gold Ultrastructure Nanocomposites Nanopores Nanospheres Porosity Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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