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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Afonso, André S. Hernández-Herrero, Manuela Roig-Sagués, Artur Xavier Mattoso, Luiz H. C. Pérez-López, Briza Merkoçi, Arben Maltez-da Costa, Marisa Faria, Ronaldo C. |
| Description | Country affiliation: Spain Author Affiliation: Afonso AS ( Nanobioelectronics & Biosensors Group, Catalan Institute of Nanotechnology, CIN2 (ICN-CSIC), Universitat Autònoma de Barcelona, 08193 Bellaterra, Catalonia, Spain.) |
| Abstract | A disposable immunosensor for Salmonella enterica subsp. enterica serovar Typhimurium LT2 (S) detection using a magneto-immunoassay and gold nanoparticles (AuNPs) as label for electrochemical detection is developed. The immunosensor is based on the use of a screen-printed carbon electrode (SPCE) that incorporates a permanent magnet underneath. Salmonella containing samples (i.e. skimmed milk) have been tested by using anti-Salmonella magnetic beads (MBs-pSAb) as capture phase and sandwiching afterwards with AuNPs modified antibodies (sSAb-AuNPs) detected using differential pulse voltammetry (DPV). A detection limit of 143 cells mL(-1) and a linear range from 10(3) to 10(6) cells mL(-1) of Salmonella was obtained, with a coefficient of variation of about 2.4%. Recoveries of the sensor by spiking skimmed milk with different quantities of Salmonella of about 83% and 94% for 1.5×10(3) and 1.5×10(5) cells mL(-1) were obtained, respectively. This AuNPs detection technology combined with magnetic field application reports a limit of detection lower than the conventional commercial method carried out for comparison purposes in skimmed milk samples. |
| ISSN | 09565663 |
| Issue Number | 1 |
| Volume Number | 40 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-02-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Conductometry Instrumentation Food Analysis Gold Immunomagnetic Separation Metal Nanoparticles Milk Microbiology Salmonella Enterica Isolation & Purification Animals Biosensing Techniques Cattle Equipment Design Equipment Failure Analysis Food Contamination Analysis Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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