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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Jiang, Hong-Xin Han, Gui-Mei Kong, De-Ming Du, Yi-Chen Huo, Yan-Fang |
| Description | Author Affiliation: Du YC ( State Key Laboratory of Medicinal Chemical Biology, Research Centre for Analytical Sciences, Nankai University, Tianjin 300071, PR China); Jiang HX ( State Key Laboratory of Medicinal Chemical Biology, Research Centre for Analytical Sciences, Nankai University, Tianjin 300071, PR China); Huo YF ( State Key Laboratory of Medicinal Chemical Biology, Research Centre for Analytical Sciences, Nankai University, Tianjin 300071, PR China); Han GM ( State Key Laboratory of Medicinal Chemical Biology, Research Centre for Analytical Sciences, Nankai University, Tianjin 300071, PR China); Kong DM ( State Key Laboratory of Medicinal Chemical Biology, Research Centre for Analytical Sciences, Nankai University, Tianjin 300071, PR China) |
| Abstract | As an isothermal nucleic acid amplification technique, strand displacement amplification (SDA) reaction has been introduced in G-quadruplex DNAzyme-based sensing system to improve the sensing performance. To further provide useful information for the design of SDA-amplified G-quadruplex DNAzyme-based sensors, the effects of nicking site number in SDA template DNA were investigated. With the increase of the nicking site number from 1 to 2, enrichment of G-quadruplex DNAzyme by SDA is changed from a linear amplification to an exponential amplification, thus greatly increasing the amplification efficiency and subsequently improving the sensing performance of corresponding sensing system. The nicking site number cannot be further increased because more nicking sites might result in high background signals. However, we demonstrated that G-quadruplex DNAzyme enrichment efficiency could be further improved by introducing a cross-triggered SDA strategy, in which two templates each has two nicking sites are used. To validate the proposed cross-triggered SDA strategy, we used it to develop a sensing platform for the detection of uracil-DNA glycosylase (UDG) activity. The sensor enables sensitive detection of UDG activity in the range of 1 × 10(-4)-1 U/mL with a detection limit of 1 × 10(-4)U/mL. |
| ISSN | 09565663 |
| Volume Number | 77 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-03-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Dna, Catalytic Chemistry G-quadruplexes Nucleic Acid Amplification Techniques Instrumentation Spectrophotometry, Ultraviolet Uracil-dna Glycosidase Analysis Dna Breaks, Single-stranded Genetics Enzyme Activation Equipment Design Equipment Failure Analysis Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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