NDLI: VEGF-conjugated alginate hydrogel prompt angiogenesis and improve pancreatic islet engraftment and function in type 1 diabetes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yin, Nina Han, Yongming Xu, Hanlin Gao, Yisen Yi, Tao Yao, Jiale Dong, Li Cheng, Dejun Chen, Zebin
Description	Country affiliation: China Author Affiliation: Yin N ( Department of Anatomy, Basic Medical College, Hubei University of Chinese Medicine, Wuhan, Hubei, China.); Han Y ( Department of Anatomy, Basic Medical College, Hubei University of Chinese Medicine, Wuhan, Hubei, China.); Xu H ( Pharmacy Faculty, Hubei University of Chinese Medicine, Wuhan, Hubei, China.); Gao Y ( Acupuncture and Moxibustion College, Hubei University of Chinese Medicine, Wuhan, Hubei, China.); Yi T ( Acupuncture and Moxibustion College, Hubei University of Chinese Medicine, Wuhan, Hubei, China.); Yao J ( Basic Medical College, Hubei University of Chinese Medicine, Wuhan, Hubei, China.); Dong L ( Basic Medical College, Hubei University of Chinese Medicine, Wuhan, Hubei, China.); Cheng D ( Basic Medical College, Hubei University of Chinese Medicine, Wuhan, Hubei, China.); Chen Z ( Acupuncture and Moxibustion College, Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, Wuhan, Hubei, China. Electronic address: chenzebin-hbtcm@outlook.com.)
Abstract	Type 1 diabetes was a life-long disease that affected numerous people around the world. Insulin therapy has its limitations that may involve hyperglycemia and heavy burden of patient by repeated dose. Islet transplantation emerged as a promising approach to reach periodical reverse of diabetes, however, transplanted islets suffer from foreign body reaction and lack of nutrition and oxygen supply, especially in the blood-vessel-shortage subcutaneous site which was preferred by patient and surgeon. In this study, we designed and synthesized a vascular endothelial growth factor (VEGF) conjugated alginate material to encapsulate the transplanted islets via 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) reaction, and successful conjugation was confirmed by Nuclear Magnetic Resonance H1 spectrum. The best VEGF concentration (100ng/ml) was determined by the combined studies of the mechanical property and endothelial cell growth assay. In vivo study, conjugated VEGF on alginate exhibited sustained promoting angiogenesis property after subcutaneous transplantation by histology study and islets encapsulated in this material achieved long term therapeutic effect (up to 50days) in the diabetic mice model. In conclusion, this study establishes a simple biomaterial strategy for islet transplantation to enhance islet survival and function, which could be a feasible therapeutic alternative for type 1 diabetes.
File Format	HTM / HTML
ISSN	09284931
Volume Number	59
e-ISSN	18730191
Journal	Materials Science and Engineering: C
Language	English
Publisher	Elsevier
Publisher Date	2016-02-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Materials Science Alginates Chemistry Diabetes Mellitus, Type 1 Surgery Hydrogel Islets Of Langerhans Transplantation Methods Neovascularization, Physiologic Drug Effects Vascular Endothelial Growth Factor A Pharmacology Animals Diabetes Mellitus, Experimental Glucuronic Acid Hexuronic Acids Islets Of Langerhans Male Mice Mice, Inbred C57bl Journal Article
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Biomaterials Condensed Matter Physics Bioengineering Mechanical Engineering

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