NDLI: Localised controlled release of simvastatin from porous chitosan-gelatin scaffolds engrafted with simvastatin loaded PLGA-microparticles for bone tissue engineering application.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Gentile, Piergiorgio Nandagiri, Vijay Kumar Daly, Jacqueline Chiono, Valeria Mattu, Clara Tonda-Turo, Chiara Ciardelli, Gianluca Ramtoola, Zebunnissa
Description	Country affiliation: United kingdom Author Affiliation: Gentile P ( Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy); Nandagiri VK ( Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy); Daly J ( Division of Biology, Department of Anatomy, Royal College of Surgeons in Ireland, 123, St. Stephen Green, Dublin 2, Ireland.); Chiono V ( Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy.); Mattu C ( Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy.); Tonda-Turo C ( Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy.); Ciardelli G ( Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy.); Ramtoola Z ( School of Pharmacy, Royal College of Surgeons in Ireland, 123, St. Stephen Green, Dublin 2, Ireland. Electronic address: zramtoola@rcsi.ie.)
Abstract	Localised controlled release of simvastatin from porous freeze-dried chitosan-gelatin (CH-G) scaffolds was investigated by incorporating simvastatin loaded poly-(dl-lactide-co-glycolide) acid (PLGA) microparticles (MSIMs) into the scaffolds. MSIMs at 10% w/w simvastatin loading were prepared using a single emulsion-solvent evaporation method. The MSIM optimal amount to be incorporated into the scaffolds was selected by analysing the effect of embedding increasing amounts of blank PLGA microparticles (BL-MPs) on the scaffold physical properties and on the in vitro cell viability using a clonal human osteoblastic cell line (hFOB). Increasing the BL-MP content from 0% to 33.3% w/w showed a significant decrease in swelling degree (from 1245±56% to 570±35%). Scaffold pore size and distribution changed significantly as a function of BL-MP loading. Compressive modulus of scaffolds increased with increasing BL-MP amount up to 16.6% w/w (23.0±1.0kPa). No significant difference in cell viability was observed with increasing BL-MP loading. Based on these results, a content of 16.6% w/w MSIM particles was incorporated successfully in CH-G scaffolds, showing a controlled localised release of simvastatin able to influence the hFOB cell proliferation and the osteoblastic differentiation after 11 days.
File Format	HTM / HTML
ISSN	09284931
Volume Number	59
e-ISSN	18730191
Journal	Materials Science and Engineering: C
Language	English
Publisher	Elsevier
Publisher Date	2016-02-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Materials Science Chitosan Gelatin Osteoblasts Metabolism Simvastatin Tissue Engineering Methods Tissue Scaffolds Chemistry Cell Line Pharmacokinetics Pharmacology Humans Materials Testing Cytology Porosity Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Biomaterials Condensed Matter Physics Bioengineering Mechanical Engineering

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