NDLI: Adhesion and internalization differences of COM nanocrystals on Vero cells before and after cell damage.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Gan, Qiong-Zhi Sun, Xin-Yuan Ouyang, Jian-Ming
Description	Country affiliation: China Author Affiliation: Gan QZ ( Department of chemistry, Jinan University, Guangzhou 510632, China); Sun XY ( Department of chemistry, Jinan University, Guangzhou 510632, China); Ouyang JM ( Department of chemistry, Jinan University, Guangzhou 510632, China)
Abstract	The adhesion and internalization between African green monkey kidney epithelial (Vero) cells (before and after oxidative damage by hydrogen peroxide) and calcium oxalate monohydrate (COM) nanocrystals (97±35nm) were investigated so as to discuss the molecular and cellular mechanism of kidney stone formation. Scanning electron microscope (SEM) was used to observe the Vero-COM nanocrystal adhesion; the nanocrystal-cell adhesion was evaluated by measuring the content of malonaldehyde (MDA), the activity of superoxide dismutase (SOD), the expression level of cell surface osteopontin (OPN) and the change of Zeta potential. Confocal microscopy and flow cytometry were used for the observation and quantitative analysis of crystal internalization. In the process of adhesion, the cell viability and the SOD activity declined, the MDA content, Zeta potential, and the OPN expression level increased. The adhesive capacity of injured Vero was obviously stronger than normal cells; in addition the injured cells promoted the aggregation of COM nanocrystals. The capacity of normal cells to internalize crystals was obviously stronger than that of injured cells. Cell injury increased adhesive sites on cell surface, thereby facilitating the aggregation of COM nanocrystals and their attachment, which results in enhanced risk of calcium oxalate stone formation.
File Format	HTM / HTML
ISSN	09284931
Volume Number	59
e-ISSN	18730191
Journal	Materials Science and Engineering: C
Language	English
Publisher	Elsevier
Publisher Date	2016-02-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Materials Science Calcium Oxalate Metabolism Nanoparticles Chemistry Animals Cell Adhesion Physiology Cercopithecus Aethiops Vero Cells Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Biomaterials Condensed Matter Physics Bioengineering Mechanical Engineering

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