| Author |
Bittencourt, Clicia Ramos de Oliveira Farias, Emanuel Airton Bezerra, Karla Costa Véras, Leiz Maria Costa Silva, Vladimir Costa Costa, Carlos Henrique Nery Bemquerer, Marcelo P. Silva, Luciano Paulino de Souza de Almeida Leite, José Roberto Eiras, Carla |
| Description |
Country affiliation: Brazil Author Affiliation: Bittencourt CR ( Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaíba, PI 64202020, Brazil.); de Oliveira Farias EA ( Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaíba, PI 64202020, Brazil.); Bezerra KC ( Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaíba, PI 64202020, Brazil.); Véras LM ( Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaíba, PI 64202020, Brazil.); Silva VC ( Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaíba, PI 64202020, Brazil); Costa CH ( Laboratório de Pesquisas em Leishmanioses, Instituto de Doenças Tropicais Natan Portela-IDTNP, Teresina 64001450, Brazil.); Bemquerer MP ( EMBRAPA Recursos Genéticos e Biotecnologia, 70770-917 Brasília, DF, Brazil); Silva LP ( EMBRAPA Recursos Genéticos e Biotecnologia, 70770-917 Brasília, DF, Brazil.); de Souza de Almeida Leite JR ( Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaíba, PI 64202020, Brazil.); Eiras C ( Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaíba, PI 64202020, Brazil) |
| Abstract |
This report details the development of thin films containing an antimicrobial peptide, specifically, dermaseptin 01 (GLWSTIKQKGKEAAIAAA-KAAGQAALGAL-NH2, [DRS 01]), and a natural polysaccharide, for a novel application in detecting the presence of Leishmania cells and maintaining anti-leishmanial activity. The peptide DRS 01 was immobilized in conjunction with natural cashew gum (CG) onto an indium tin oxide (ITO) substrate using the Layer-by-Layer (LbL) deposition technique. The LbL film ITO/CG/DRS 01, containing DRS 01 as the outer layer, was capable of detecting the presence of Leishmania cells and acting as an anti-leishmanial system. Detection was performed using cyclic voltammetry (CV) in phosphate buffer (pH7.2) in the presence of promastigote cells (0-10(7)cells/mL). The results showed a linear and inversely proportional relation between the concentration of Leishmania infantum protozoan cells and the measured current values obtained for the films, which was attributed to the effect of peptide-induced lysis of the cell membrane, and resulted in freed residues that were adsorbed on the electrode surface. With this, the paper shows a method using thin films with this new material to demonstrate the anti-leishmanial activity in vitro models of carpet-like mechanisms. |
