NDLI: Eggshell membrane: A natural substrate for immobilization and detection of DNA.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ray, Preetam Guha Roy, Somenath
Description	Country affiliation: India Author Affiliation: Ray PG ( Sensor & Actuator Division, CSIR - Central Glass and Ceramic Research Institute, Kolkata 700032, India.); Roy S ( Sensor & Actuator Division, CSIR - Central Glass and Ceramic Research Institute, Kolkata 700032, India. Electronic address: sroy@cgcri.res.in.)
Abstract	Chemically modified eggshell membranes (ESM) have been explored as potentially novel platforms for immobilization of oligonucleotides and subsequent detection of target DNA. The fibrous network of the native ESM as well those functionalized with acetic acid or n-butyl acetate has been examined by field-emission scanning electron microscopy (FESEM). The formation of surface functional moieties has been confirmed by Fourier-transform infrared spectroscopy (FTIR). DNA molecules, with an end terminal -NH2 group (at 5' end) have been immobilized on the chemically modified ESM surface. The effect of surface modification on the DNA immobilization efficiency has been investigated using fluorescence microscopy and atomic force microscopy (AFM). The above studies concurrently suggest that functionalization of ESM with n-butyl acetate causes a better homogeneity of the DNA probes on the membrane surface. On-chip hybridization of the target DNA with the surface bound capture probes has been performed on the functionalized membranes. It is observed that n-butyl acetate modification of ESM pushes the limit of detection (LOD) of the DNA sensors by at least an order of magnitude compared to the other modification method.
File Format	HTM / HTML
ISSN	09284931
Volume Number	59
e-ISSN	18730191
Journal	Materials Science and Engineering: C
Language	English
Publisher	Elsevier
Publisher Date	2016-02-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Materials Science Dna Analysis Chemistry Egg Shell Animals Chickens Ultrastructure Microscopy, Electron, Scanning Spectroscopy, Fourier Transform Infrared Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Biomaterials Condensed Matter Physics Bioengineering Mechanical Engineering

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