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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kwak, Ji-Yun Kim, Min-Gyu Kim, Young-Wan Ban, Hyun-Seung Won, Mi-Sun Park, Jong-Tae Park, Kwan-Hwa |
| Description | Author Affiliation: Kwak JY ( Department of Food Science and Technology, Chungnam National University, Daejeon 305-764, Republic of Korea.); Kim MG ( Department of Food and Biotechnology, Korea University, Sejong 339-700, Republic of Korea.); Kim YW ( Department of Food and Biotechnology, Korea University, Sejong 339-700, Republic of Korea.); Ban HS ( Biomedical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.); Won MS ( Biomedical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.); Park JT ( Department of Food Science and Technology, Chungnam National University, Daejeon 305-764, Republic of Korea. Electronic address: jtpark@cnu.ac.kr.); Park KH ( Department of Food Science and Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea.) |
| Abstract | Escherichia coli mutant TBP38 lacks glycogen synthase (GlgA) and maltodextrin phosphorylase (MalP). When grown on maltose in fed-batch fermentation TBP38 accumulated more than 50-fold higher glycogen-type polysaccharide than its parental strain. The polysaccharides were extracted at different growth stages and migrated as one peak in size-exclusion chromatography. TBP38 produced polysaccharides ranging 2.6 × 10(6)-4.6 × 10(6)Da. A ratio of short side-chains (DP ⦠12) in the polysaccharides was greater than 50%, and number-average degree of polymerization varied from 9.8 to 8.4. The polysaccharides showed 70-290 times greater water-solubility than amylopectin. Km values using porcine and human pancreatic -amylases with polysaccharides were 2- to 4-fold larger than that of amylopectin. kcat values were similar for both -amylases. The TBP38 polysaccharides had 40-60% lower digestibility to amyloglucosidase than amylopectin. Intriguingly, the polysaccharides showed strong immunostimulating effects on mouse macrophage cell comparable to lipopolysaccharides. The lipopolysaccharide contamination levels were too low to account for this effect. |
| File Format | HTM / HTML |
| ISSN | 01448617 |
| Volume Number | 136 |
| e-ISSN | 18791344 |
| Journal | Carbohydrate Polymers |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-01-20 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry__semicolon__materials Discipline Science Glucosyltransferases Genetics Glycogen Synthase Immunologic Factors Chemistry Polysaccharides, Bacterial Animals Cell Line Escherichia Coli Metabolism Deficiency Hydrolysis Pharmacology Macrophages Drug Effects Mice Mutation Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Materials Chemistry Polymers and Plastics |
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