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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Moniz, Patrícia Ho, Ai Ling Duarte, Luís C. Kolida, Sofia Rastall, Robert A. Pereira, Helena Carvalheiro, Florbela |
| Description | Country affiliation: Portugal Author Affiliation: Moniz P ( Unidade de Bioenergia, LNEG - Laboratório Nacional de Energia e Geologia, Estrada do Paço do Lumiar, 22, Lisboa 1649-038, Portugal); Ho AL ( Department of Food and Nutritional Sciences, University of Reading, Whiteknights, P.O. Box 226, Reading RG6 6AP, United Kingdom); Duarte LC ( Unidade de Bioenergia, LNEG - Laboratório Nacional de Energia e Geologia, Estrada do Paço do Lumiar, 22, Lisboa 1649-038, Portugal.); Kolida S ( Department of Food and Nutritional Sciences, University of Reading, Whiteknights, P.O. Box 226, Reading RG6 6AP, United Kingdom.); Rastall RA ( Department of Food and Nutritional Sciences, University of Reading, Whiteknights, P.O. Box 226, Reading RG6 6AP, United Kingdom.); Pereira H ( Centro de Estudos Florestais, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal.); Carvalheiro F ( Unidade de Bioenergia, LNEG - Laboratório Nacional de Energia e Geologia, Estrada do Paço do Lumiar, 22, Lisboa 1649-038, Portugal. Electronic address: florbela.carvalheiro@lneg.pt.) |
| Abstract | This work evaluates the bifidogenic potential of substituted xylo-oligosaccharides (XOS) obtained from a lignocellulosic feedstock (corn straw). Autohydrolysis was used to selectively hydrolyse the xylan-rich hemicellulosic fraction and the soluble oligosaccharides were purified by gel filtration chromatography. Selected oligosaccharides fractions within the target ranges of polymerization degree (4-6 and 9-21, samples S1 and S2, respectively) were characterized and their bifidogenic potential was investigated by in vitro fermentations using human fecal inocula. Bacterial growth was assessed by fluorescent in situ hybridization (FISH). XOS consumption and short-chain fatty acids (SCFA) production were evaluated and compared with commercial oligosaccharides. Under the tested conditions, all the substrates were utilized by the microbiota, and fermentation resulted in increased bifidobacteria populations. Samples S1 and S2 increased bifidobacteria populations and the production profile of SCFA was similar for XOS samples and commercial oligosaccharides although XOS samples displayed the highest concentration of SCFA on longer fermentation times. |
| File Format | HTM / HTML |
| ISSN | 01448617 |
| Volume Number | 136 |
| e-ISSN | 18791344 |
| Journal | Carbohydrate Polymers |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-01-20 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry__semicolon__materials Discipline Science Cellulose Chemistry Fermentation Glucuronates Industrial Microbiology Methods Oligosaccharides Zea Mays Bifidobacterium Growth & Development Isolation & Purification Metabolism Feces Microbiology Humans Hydrolysis Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Materials Chemistry Polymers and Plastics |
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