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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Luo, Y. Yobas, L. |
| Copyright Year | 2015 |
| Description | Author affiliation: Dept. of Med. Eng., California Inst. of Technol., Pasadena, CA, USA (Luo, Y.) || Dept. of Electron. & Comput. Eng., Hong Kong Univ. of Sci. & Technol., Hong Kong, China (Yobas, L.) |
| Abstract | We present microfluidic designs for dielectrophoretic positioning of single cells and their subsequent analysis based on 3D silicon electrodes featuring built-in capillaries. The designs are presented through numerical simulations as well as experimental validations showing effectiveness of dielectrophoretic forces in trapping single cells. Single-cell experiments are also demonstrated including viability assays and ionophore-mediated intracellular calcium flux assays. Seal resistance measurements between the capillaries and cells trapped, which are essential for patch-clamp assays, are reported. |
| Starting Page | 540 |
| Ending Page | 543 |
| File Size | 4494272 |
| Page Count | 4 |
| File Format | |
| ISBN | 9781479989553 |
| ISSN | 21641641 |
| DOI | 10.1109/TRANSDUCERS.2015.7180980 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2015-06-21 |
| Publisher Place | USA |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Electrodes Silicon Three-dimensional displays Glass Dielectrophoresis Charge carrier processes Electric fields microcapillaries Single-cell analysis dielectrophoresis 3D microelectrodes |
| Content Type | Text |
| Resource Type | Article |
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