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Content Provider | IEEE Xplore Digital Library |
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Author | Kin Fong Lei Che-Wei Hsu Shing-I Yang Cheng-Yuan Lin Min-Hsien Wu |
Copyright Year | 2012 |
Description | Author affiliation: Graduate Institute of Medical Mechatronics, Chang Gung University, Taoyuan, Taiwan (Kin Fong Lei; Che-Wei Hsu; Shing-I Yang) || Graduate Institute of Biochemical and Biomedical, Chang Gung University, Taoyuan, Taiwan (Cheng-Yuan Lin; Min-Hsien Wu) |
Abstract | Onsite determination of cell concentration in 3D cell culture construct is proposed based on electrical impedance measurement through on-chip vertical electrodes embedded in the 3D cell culture chamber. Cells were encapsulated in agarose gel and cultured in the 3D cell culture chamber. A pair of vertical electrodes was fabricated at the opposite sidewalls of the culture chamber for performing electrical impedance measurement. Electric field penetrated through the cells/agarose construct and the cell concentration in the construct can be estimated by the impedance change. In this study, cell concentrations of $10^{4},$ $10^{5},$ and $10^{6}$ cells/ml can be determined directly without altering the cell property. |
Starting Page | 1 |
Ending Page | 4 |
File Size | 745773 |
Page Count | 4 |
File Format | |
ISBN | 9781457717666 |
ISSN | 19300395 |
e-ISBN | 9781457717673 |
e-ISBN | 9781457717659 |
DOI | 10.1109/ICSENS.2012.6411554 |
Language | English |
Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
Publisher Date | 2012-10-28 |
Publisher Place | Taiwan |
Access Restriction | Subscribed |
Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
Subject Keyword | Impedance Electrodes Impedance measurement System-on-a-chip Glass Resistance Frequency measurement |
Content Type | Text |
Resource Type | Article |
Subject | Electrical and Electronic Engineering |
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