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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Tae Goo Kang Hong Miao Ji Tan, S.P.M. Tay, G.K.I. Ang, M.Y.D. Rafei, S.R.B.M. Kum Cheong Tang Li Zhang Xiaowu Zhang Yu Chen |
| Copyright Year | 2010 |
| Description | Author affiliation: Institute of Microelectronics, A*STAR (Agency for Science, Technology and Research), Singapore (Tae Goo Kang; Hong Miao Ji; Tan, S.P.M.; Tay, G.K.I.; Ang, M.Y.D.; Rafei, S.R.B.M.; Kum Cheong Tang; Li Zhang; Xiaowu Zhang; Yu Chen) |
| Abstract | This paper presents a microelectrothermofluidic packaging of integrated microdevice for viral ribonucleic acid (RNA) extraction from blood samples and its amplification through a reverse transcription (RT)-polymerase chain reaction (PCR) method for application to the point-of-care (POC) infectious disease diagnostics. Integrated microdevice consists of solid phase extraction microchannel structure, microfluidic mixer, and meander-shaped microchamber for RT-PCR. Metallic microheater and resistive temperature detection (RTD) microsenosr is monolithically integrated with fluidic components. In order to secure both electrical and fluidic interconnections, spring-loaded electrical pin and drilled microchannel structure has been design in polycarbonate micropackaging structure. In the biotesting, we have demonstrated to detect dengue serotype III virus from the 80µl of blood sample with 80pfu virus concentration. |
| Starting Page | 13 |
| Ending Page | 16 |
| File Size | 1614304 |
| Page Count | 4 |
| File Format | |
| ISBN | 9781424485604 |
| e-ISBN | 9781424485628 |
| e-ISBN | 9781424485611 |
| DOI | 10.1109/EPTC.2010.5702597 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2010-12-08 |
| Publisher Place | Singapore |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Silicon Packaging Blood RNA Microchannel Substrates Microfluidics |
| Content Type | Text |
| Resource Type | Article |
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