NDLI: Electrochemical biosensor based on modified graphene oxide for tuberculosis diagnosis

Content Provider	IEEE Xplore Digital Library
Author	Pei Zhang Xiaosen Chai Chun Xu Jia Zhou
Copyright Year	2011
Description	Author affiliation: State Key Laboratory of ASIC & System, Department of Microelectronics, Fudan University, Shanghai 200433, China (Pei Zhang; Xiaosen Chai; Chun Xu; Jia Zhou)
Abstract	In this paper, graphene oxide (GO) was modified in-situ on an electrochemical electrode array for attachment of protein. GO suspension made from natural graphite flakes was directly dip-coated onto the Au working electrodes. Detected by cyclic voltammetry, the peak current of GO on Au electrode increased by 1µA as compared with bare gold electrode. Modified graphgene oxide (MGO) with stable active esters was formed by in-situ modifying GO with N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDAC)/ N-hydroxysuccinimide (NHS). MGO was ready for protein attachment. An immunesensor chip (GO-antigen) was prepared by tuberculosis (TB) antigens bound with MGO. The peak of CV curve of GO-antigen increased by about 3µA as compared with that of MGO, however, after the GO-antigen reaction with the antibody, the peak of the current decreased 4µA. Such results showed potential applications of the immunosensor.
Starting Page	653
Ending Page	656
File Size	519572
Page Count	4
File Format	PDF
ISBN	9781612841922
ISSN	2162755X
e-ISBN	9781612841939
e-ISBN	9781612841915
DOI	10.1109/ASICON.2011.6157290
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-10-25
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Immune system Biosensors Fluorescence Gold Microscopy Immunosensor Graphene oxide Biosensor Electrochemistry
Content Type	Text
Resource Type	Article

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