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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Chen, Wei Ogabiela, Edward Wu, Yucheng Cui, Jiewu Adeloju, Samuel B. |
| Description | Country affiliation: Australia Author Affiliation: Ogabiela E ( NanoScience and Sensor Technology Research Group, School of Chemistry, Monash University, Clayton, Victoria 3800, Australia.); Adeloju SB ( NanoScience and Sensor Technology Research Group, School of Chemistry, Monash University, Clayton, Victoria 3800, Australia. Electronic address: Sam.Adeloju@monash.edu.); Cui J ( Laboratory for Functional Nanomaterials and Devices, School of Materials Science and Engineering, Hefei University of Technology, Hefei 230009, Anhui, China.); Wu Y ( Laboratory for Functional Nanomaterials and Devices, School of Materials Science and Engineering, Hefei University of Technology, Hefei 230009, Anhui, China.); Chen W ( School of Biotechnology & Food Engineering, Hefei University of Technology, Hefei 230009, Anhui, China.) |
| Abstract | A novel phosphate amperometric nanobiosensor, based on an intimate integration of pyruvate oxidase (PyOx) and its cofactors, thiamine pyrophosphate (TPP) and flavin adenine dinucleotide (FAD), with a highly ordered gold nanowires array (AuNWA) has been developed. The successful integration of PyOx and the co-factors, via crosslinking with bovine serum albumin (BSA) and glutaraldehyde (GLA), onto the AuNWA was confirmed by cyclic voltammetry and amperometry. The resulting nanobiosensor achieved a detection limit of 0.1 µM, a linear concentration range of 12.5-1000 µM, and a sensitivity of 140.3 µA mM(-1)cm(-2). Notably, the incorporation of the AuNWA reduced the required PyOx concentration by 70-120 fold and the presence of common interferants, such as chloride, sulfate, fluoride, nitrite and nitrate ions did not interfere with phosphate detection. Furthermore, the nanobiosensor demonstrated a very high stability with repeated use over two weeks and was successfully used for the determination of phosphate in water samples with an average recovery of 96.6 ± 4.9%. |
| ISSN | 09565663 |
| Volume Number | 71 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-09-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Conductometry Instrumentation Gold Chemistry Nanowires Phosphates Analysis Pyruvate Oxidase Water Pollutants, Chemical Biosensing Techniques Electrodes Environmental Monitoring Enzymes, Immobilized Equipment Design Equipment Failure Analysis Metal Nanoparticles Ultrastructure Molecular Conformation Nanotechnology Reproducibility Of Results Sensitivity And Specificity Systems Integration Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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