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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Li, Jun Guan, Yifu Zhao, Guojie Hu, Tianyu Wei, Hua Shang, Hong |
| Description | Country affiliation: China Author Affiliation: Zhao G ( Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, Liaoning 110001, China.); Hu T ( Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, Liaoning 110001, China.); Li J ( Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, Liaoning 110001, China.); Wei H ( Animal Science and Veterinary Medicine College, Shenyang Agricultural University, Shenyang, Liaoning 110866, China.); Shang H ( Department of Clinical Diagnosis, the First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, China.); Guan Y ( Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, Liaoning 110001, China. Electronic address: yfguan@mail.cmu.edu.cn.) |
| Abstract | Rolling circle amplification (RCA) has been considered as a powerful tool for nucleic acids detection. Here, a novel repressor-RCA-based method for L-tryptophan (L-Trp) detection was developed. This method utilizes the specific interaction between the RCA circular template and the Trp repressor protein (TrpR) involved in trp operon of Escherichia coli (E. coli). In the absence of L-Trp, the TrpR protein could not bind to the RCA template, and the RCA process can be continued. When L-Trp is present, the activated TrpR will bind to the operon sequence on the RCA template and inhibit the RCA reaction. Thus, the concentration of L-Trp is correlated directly with the fluorescent RCA signals. We succeeded in detecting L-Trp in a single step in simple homogeneous reaction system. The detection limit was estimated to be 0.77 µM (S/N=3) with good linearity. The method can unambiguously distinguish L-Trp from other 19 standard amino acids and L-Trp analogs. This strategy is also promising for detecting many small molecules such as other amino acids and carbohydrates. |
| ISSN | 09565663 |
| Volume Number | 71 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-09-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Bacterial Proteins Metabolism Biosensing Techniques Methods Escherichia Coli Repressor Proteins Tryptophan Analysis Allosteric Regulation Base Sequence Binding Sites Limit Of Detection Models, Molecular Operon Spectrometry, Fluorescence Genetics Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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