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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Patskovsky, Sergiy Dallaire, Anne-Marie Meunier, Michel Vallée-Bélisle, Alexis |
| Description | Author Affiliation: Dallaire AM ( Laser Processing and Plasmonics Laboratory, École Polytechnique de Montréal, Department of Engineering Physics, C.P. 6079, succ. Centre-Ville, Montréal, QC, Canada H3C 3A7.); Patskovsky S ( Laser Processing and Plasmonics Laboratory, École Polytechnique de Montréal, Department of Engineering Physics, C.P. 6079, succ. Centre-Ville, Montréal, QC, Canada H3C 3A7.); Vallée-Bélisle A ( Laboratory of Biosensors and Nanomachines, Université de Montréal, Department of Chemistry, C.P. 6128, succ. Centre-Ville, Montréal, QC, Canada H3C 3J7. Electronic address: a.vallee-belisle@umontreal.ca.); Meunier M ( Laser Processing and Plasmonics Laboratory, École Polytechnique de Montréal, Department of Engineering Physics, C.P. 6079, succ. Centre-Ville, Montréal, QC, Canada H3C 3A7. Electronic address: michel.meunier@polymtl.ca.) |
| Abstract | In this paper, we present the development of a nanoswitch-based electrochemical surface plasmon resonance (eSPR) transducer for the multiplexed and selective detection of DNA and other biomolecules directly in complex media. To do so, we designed an experimental set-up for the synchronized measurements of electrochemical and electro-plasmonic responses to the activation of multiple electrochemically labeled structure-switching biosensors. As a proof of principle, we adapted this strategy for the detection of DNA sequences that are diagnostic of two pathogens (drug-resistant tuberculosis and Escherichia coli) by using methylene blue-labeled structure-switching DNA stem-loop. The experimental sensitivity of the switch-based eSPR sensor is estimated at 5 nM and target detection is achieved within minutes. Each sensor is reusable several times with a simple 8M urea washing procedure. We then demonstrated the selectivity and multiplexed ability of these switch-based eSPR by simultaneously detecting two different DNA sequences. We discuss the advantages of the proposed eSPR approach for the development of highly selective sensor devices for the rapid and reliable detection of multiple molecular markers in complex samples. |
| ISSN | 09565663 |
| Volume Number | 71 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-09-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Dna, Bacterial Analysis Electrochemical Techniques Instrumentation Escherichia Coli Genetics Methylene Blue Chemistry Mycobacterium Tuberculosis Oligonucleotides Surface Plasmon Resonance Base Sequence Blood Equipment Design Isolation & Purification Escherichia Coli Infections Diagnosis Microbiology Humans Models, Molecular Nucleic Acid Conformation Oxidation-reduction Tuberculosis Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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