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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Li, Yunqiu Zhang, Yawen Wu, Wenjian Jiang, Yuren Hu, Biru |
| Description | Author Affiliation: Zhang Y ( College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China); Li Y ( Department of Chemistry and Biology, College of Science, National University of Defense Technology, Changsha 410073, PR China. Electronic address: liyunqiu83@aliyun.com.cn.); Wu W ( Department of Chemistry and Biology, College of Science, National University of Defense Technology, Changsha 410073, PR China. Electronic address: wjwu67@126.com.); Jiang Y ( College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China. Electronic address: jiangyr@mail.csu.edu.cn.); Hu B ( Department of Chemistry and Biology, College of Science, National University of Defense Technology, Changsha 410073, PR China. Electronic address: brhu@yeah.net.) |
| Abstract | A glucose biosensor was developed via direct immobilization of glucose oxidase (GOD) by self-assembled cysteamine monolayer on Au electrode surface followed by coating chitosan on the surface of electrode. In this work, chitosan film was coated on the surface of GOD as a protection film to ensure the stability and biocompatibility of the constructed glucose biosensor. The different application ranges of sensors were fabricated by immobilizing varied layers of GOD. The modified surface film was characterized by a scanning electron microscope (SEM) and the fabrication process of the biosensor was confirmed through electrochemical impedance spectroscopy (EIS) of ferrocyanide. The performance of cyclic voltammetry (CV) in the absence and presence of 25 mM glucose and ferrocenemethanol showed a diffusion-controlled electrode process and reflected the different maximum currents between the different GOD layers. With the developed glucose biosensor, the detection limits of the two linear responses are 49.96 µM and 316.8 µM with the sensitivities of 8.91 µA mM(-1)cm(-2) and 2.93 µA mM(-1)cm(-2), respectively. In addition, good stability (up to 30 days) of the developed biosensor was observed. The advantages of this new method for sensors construction was convenient and different width ranges of detection can be obtained by modified varied layers of GOD. The sensor with two layers of enzyme displayed two current linear responses of glucose. The present work provided a simplicity and novelty method for producing biosensors, which may help design enzyme reactors and biosensors in the future. |
| ISSN | 09565663 |
| Volume Number | 60 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-10-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Chitosan Chemistry Conductometry Cysteamine Electrodes Glucose Oxidase Glucose Analysis Coated Materials, Biocompatible Chemical Synthesis Enzymes, Immobilized Equipment Design Equipment Failure Analysis Gold Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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