NDLI: An aptameric graphene nanosensor for label-free detection of small-molecule biomarkers.

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Rapid, sensitive, and selective fluorescent DNA detection using iron-based metal-organic framework nanorods: Synergies of the metal center and organic linker.

SERS-active Au@Ag nanorod dimers for ultrasensitive dopamine detection.

An insertion approach electrochemical aptasensor for mucin 1 detection based on exonuclease-assisted target recycling.

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A fluorescent biosensing platform based on the polydopamine nanospheres intergrating with Exonuclease III-assisted target recycling amplification.

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Novel electrochemical dual-aptamer-based sandwich biosensor using molybdenum disulfide/carbon aerogel composites and Au nanoparticles for signal amplification.

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Electrochemical detection of Bisphenol A with high sensitivity and selectivity using recombinant protein-immobilized graphene electrodes.

An aptameric graphene nanosensor for label-free detection of small-molecule biomarkers.

Replacing antibodies with aptamers in lateral flow immunoassay.

A simple and rapid label-free fluorimetric biosensor for protamine detection based on glutathione-capped CdTe quantum dots aggregation.

An amplified electrochemical strategy using DNA-QDs dendrimer superstructure for the detection of thymine DNA glycosylase activity.

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Electrochemiluminescence device for in-situ and accurate determination of CA153 at the MCF-7 cell surface based on graphene quantum dots loaded surface villous Au nanocage.

Detection of Interferon gamma using graphene and aptamer based FET-like electrochemical biosensor.

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In situ formation of graphene layers on graphite surfaces for efficient anodes of microbial fuel cells.

An ultrasensitive electrochemical immunosensor for apolipoprotein E4 based on fractal nanostructures and enzyme amplification.

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An aptameric graphene nanosensor for label-free detection of small-molecule biomarkers.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Nuckolls, Colin Lin, Qiao Kim, Jinho Hone, James Yu, Jaeeun Wang, Cheng Lee, Sunwoo Zhu, Yibo Lee, Gwan-Hyoung Liu, Guohua Yang, Jaeyoung Pei, Renjun
Description	Country affiliation: China Author Affiliation: Wang C ( Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA); Kim J ( Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA.); Zhu Y ( Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA.); Yang J ( Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA.); Lee GH ( Department of Materials Science and Engineering, Yonsei University, Seoul 120749, Republic of Korea.); Lee S ( Department of Electrical Engineering, Columbia University, New York, NY 10027, USA.); Yu J ( Department of Chemistry, Columbia University, New York, NY 10027, USA.); Pei R ( Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu 215123, China.); Liu G ( Department of Microelectronic Engineering, Nankai University, Tianjin 300071, China.); Nuckolls C ( Department of Chemistry, Columbia University, New York, NY 10027, USA.); Hone J ( Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA.); Lin Q ( Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA. Electronic address: qlin@columbia.edu.)
Abstract	This paper presents an aptameric graphene nanosensor for detection of small-molecule biomarkers. To address difficulties in direct detection of small molecules associated with their low molecular weight and electrical charge, we incorporate an aptamer-based competitive affinity assay in a graphene field effect transistor (FET), and demonstrate the utility of the nanosensor with dehydroepiandrosterone sulfate (DHEA-S), a small-molecule steroid hormone, as the target analyte. In the competitive affinity assay, DHEA-S specifically binds to aptamer molecules pre-hybridized to their complementary DNA anchor molecules immobilized on the graphene surface. This results in the competitive release of the strongly charged aptamer from the DNA anchor and hence a change in electrical properties of the graphene, which can be measured to achieve the detection of DHEA-S. We present experimental data on the label-free, specific and quantitative detection of DHEA-S at clinically appropriate concentrations with an estimated detection limit of 44.7 nM, and analyze the trend observed in the experiments using molecular binding kinetics theory. These results demonstrate the potential of our nanosensor in the detection of DHEA-S and other small molecules in biomedical applications.
ISSN	09565663
e-ISSN	18734235
DOI	10.1016/j.bios.2015.04.025
Journal	Biosensors and Bioelectronics
Volume Number	71
Language	English
Publisher	Elsevier
Publisher Date	2015-09-15
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Aptamers, Nucleotide Chemistry Biosensing Techniques Instrumentation Dehydroepiandrosterone Sulfate Graphite Lab-on-a-chip Devices Equipment Design Limit Of Detection Evaluation Studies Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry

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