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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Rezaei, B. Kazemifard, N. Ensafi, Ali A. |
| Description | Author Affiliation: Ensafi AA ( Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran. Electronic address: Ensafi@cc.iut.ac.ir.); Kazemifard N ( Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.); Rezaei B ( Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.) |
| Abstract | A novel fluorescent biosensor is developed, based on glutathione-capped CdTe quantum dots aggregation, for the determination of trace amount of an important drug, protamine. In this method with increasing the protamine concentration, the fluorescence of the quantum dots was quenched due to their aggregation. Different parameters affect the sensitivity, such as pH and the amount of the quantum dots, were optimized. Using the new optical biosensor, under the optimized conditions, protamine could be measured in the range of 2.0-200 ng mL(-1) with a detection limit of 1.0 ng mL(-)(1). The relative standard deviation for five replicates determination of 30.0 ng mL(-)(1) protamine was 1.26%. The influence of common interfering species on the protamine detection was studied. The results showed that the biosensor is highly selective and sensitive for the detection of protamine. The optical biosensor was successfully used for the determination of protamine in real samples. |
| ISSN | 09565663 |
| Volume Number | 71 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-09-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Methods Cadmium Compounds Chemistry Glutathione Heparin Antagonists Blood Protamines Quantum Dots Tellurium Analysis Humans Limit Of Detection Ultrastructure Spectrometry, Fluorescence Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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