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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Su, Wenqiong Ding, Xianting Li, Zonglin Liu, Shuopeng |
| Description | Author Affiliation: Liu S ( Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, PR China.); Su W ( Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, PR China.); Li Z ( Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, PR China.); Ding X ( Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, PR China. Electronic address: dingxianting@sjtu.edu.cn.) |
| Abstract | Recent reports have indicated that aberrant expression of microRNAs is highly correlated with occurrence of lung cancer. Therefore, highly sensitive detection of lung cancer specific microRNAs provides an attractive approach in lung cancer early diagnostics. Herein, we designed 3D DNA origami structure that enables electrochemical detection of lung cancer related microRNAs. The 3D DNA origami structure is constituted of a ferrocene-tagged DNA of stem-loop structure combined with a thiolated tetrahedron DNA nanostructure at the bottom. The top portion hybridized with the lung cancer correlated microRNA, while the bottom portion was self-assembled on gold disk electrode surface, which was modified with gold nanoparticles (Au NPs) and blocked with mercaptoethanol (MCH). The preparation process and the performance of the proposed electrochemical genosensor were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Under the optimal conditions, the developed genosensor had a detection limit of 10 pM and a good linearity with microRNA concentration ranging from 100 pM to 1 µM, which showed a great potential in highly sensitive clinical cancer diagnosis application. |
| ISSN | 09565663 |
| Volume Number | 71 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-09-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Immobilized Nucleic Acids Chemistry Lung Neoplasms Genetics Micrornas Analysis Nanostructures Nucleic Acid Hybridization Electrochemical Techniques Equipment Design Ferrous Compounds Gold Humans Limit Of Detection Lung Metabolism Pathology Diagnosis Metal Nanoparticles Ultrastructure Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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