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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Li, Wen-You Wang, Yi-Zhi Yang, Ya-Qiong Zhang, Yu-Kui He, Xi-Wen |
| Description | Country affiliation: China Author Affiliation: Yang YQ ( State Key Laboratory of Medicinal Chemical Biology, and Department of Chemistry, Nankai University, Tianjin 300071, China); He XW ( State Key Laboratory of Medicinal Chemical Biology, and Department of Chemistry, Nankai University, Tianjin 300071, China); Wang YZ ( State Key Laboratory of Medicinal Chemical Biology, and Department of Chemistry, Nankai University, Tianjin 300071, China); Li WY ( State Key Laboratory of Medicinal Chemical Biology, and Department of Chemistry, Nankai University, Tianjin 300071, China); Zhang YK ( State Key Laboratory of Medicinal Chemical Biology, and Department of Chemistry, Nankai University, Tianjin 300071, China) |
| Abstract | A novel epitope molecularly imprinted polymer (EMIP) for specific recognition and direct fluorescent quantification of the target protein bovine serum albumin (BSA) was demonstrated where polymerization was performed on the surface of silica nanospheres embedded CdTe quantum dots (QDs). The synthetic peptide derived from the surface-exposed C-terminus of bovine serum albumin (BSA, residues 599-607) was selected as the template molecule. The resulting EMIP film was able to selectively capture the template peptide and the corresponding target protein BSA via the recognition cavities. Based on the fluorescence quenching, the EMIP-coated QDs (molecular imprinted polymer coating CdTe QDs using epitope as the template) nanospheres were successfully applied to the direct fluorescence quantification of BSA. Compared with BMIP-coated QDs (molecular imprinted polymer coating CdTe QDs using BSA as the template), the imprinting factor and adsorption capacity of EMIP-coated QDs were greatly increased. The prepared EMIP-coated QDs can also discriminate even one mismatched sequences from the original sequences of the epitope of the BSA. The practical analytical performance of the EMIP-coated QDs was examined by evaluating the detection of BSA in the bovine calf serum sample with satisfactory results. In addition, the resulting EMIP-coated QDs nanospheres were also successfully applied to separating BSA from the bovine blood sample. |
| ISSN | 09565663 |
| Volume Number | 54 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-04-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Cadmium Compounds Chemistry Polymers Quantum Dots Serum Albumin, Bovine Analysis Tellurium Animals Biosensing Techniques Methods Cattle Limit Of Detection Polymerization Ultrastructure Silicon Dioxide Spectrometry, Fluorescence Surface Properties Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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