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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wu, Yanfang Chen, Xi Lin, Chunshui Chen, Dongmei Luo, Feng |
| Description | Country affiliation: China Author Affiliation: Lin C ( Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.); Wu Y ( Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.); Luo F ( Fujian Research Institute of Metric Science, Fuzhou 350003, China.); Chen D ( Fujian Research Institute of Metric Science, Fuzhou 350003, China.); Chen X ( Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China) |
| Abstract | In this work, using methylene blue (MB) as a redox marker and exonuclease III (Exo III) as an amplificatory enzyme, we developed a facile and a label-free electrochemical method for sensitive DNA detection. A double-stranded DNA (dsDNA) probe was prepared by hybridizing two single-stranded DNA (ssDNA) probes. In the ssDNA probes, one ssDNA was guanine bases free and the other one consisted of many unbound guanine bases. MB could be absorbed on the unbound guanine bases owing to the specific interaction between MB and the guanine bases. When the dsDNA probe was challenged with target DNA, it induced a simple Exo III assisted cleavage process, accompanied by the release of the unbound guanine bases. Thus, the amount of MB absorbed on the electrode was much less compared to the initial signal. The detection limit for DNA was found to be as low as 20 fM. Moreover, it could discriminate mismatched DNA from perfectly matched target DNA. This detection method is simple in design, fast in operation and can be applied to detect different DNA sequences. |
| ISSN | 09565663 |
| Volume Number | 59 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-09-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Methods Dna Analysis Electrochemical Techniques Exodeoxyribonucleases Metabolism Methylene Blue Chemistry Base Sequence Genetics Dna, Viral Humans Influenza A Virus, H1n1 Subtype Isolation & Purification Influenza, Human Virology Limit Of Detection Oxidation-reduction Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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