NDLI: Amplified Chemiluminescence Detection of DNA by Strand-Displacement Polymerization Target Recycling and G-quadruplexes/Hemin DNAzyme Catalysis

Content Provider	Springer Nature Link
Author	Zhao, Limin Huang, Yong Zhang, Liangliang Zhao, Shulin
Copyright Year	2014
Abstract	A new strategy based on strand-displacement polymerization target recycling and G-quadruplex DNAzyme catalysis was developed for amplified chemiluminescence detection of DNA. The amplified detection was achieved by using the system consisted of hairpin DNA probe, G-rich DNA, primer, and polymerase Klenow fragment exo$^{−}$. When the target DNA was introduced the system, the hairpin structure of DNA probe was opened by the hybridizing of target DNA with its complementary sequence, the primer hybridized then with DNA probe and initiated polymerase-aided strand-displacement polymerization reaction, resulting in the release of target DNA and G-rich DNA. The released target DNA again hybridizes with another DNA probe to trigger the next polymerase-aided strand-displacement polymerization, generating large numbers of G-rich DNA. The G-rich DNA assembles with hemin to form the G-quadruplexes/hemin DNAzyme, which can catalyze oxidation of luminol by H$_{2}$O$_{2}$, generating chemiluminescence. This unique amplifying strategy gives a detection limit down to 2.5 pM, which is at least two to three orders of magnitude lower than that of unamplified DNA detection methods.
Starting Page	1155
Ending Page	1161
Page Count	7
File Format	PDF
ISSN	15571955
Journal	Plasmonics
Volume Number	9
Issue Number	5
e-ISSN	15571963
Language	English
Publisher	Springer US
Publisher Date	2014-05-20
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Signal-amplifying DNA Strand-displacement polymerization Chemiluminescence G-quadruplexes/hemin DNAzyme Biotechnology Nanotechnology Biophysics and Biological Physics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Biochemistry Biophysics Biotechnology

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