NDLI: Non-destructive cryo micro CT imaging enables structural and molecular analysis of human lung tissue.

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Non-destructive cryo micro CT imaging enables structural and molecular analysis of human lung tissue.

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Non-destructive cryo micro CT imaging enables structural and molecular analysis of human lung tissue.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Vasilescu, Dragos Mihai Phillion, André B. Tanabe, Naoya Kinose, Daisuke Paige, David F. Kantrowitz, Jacob J. Liu, Gang Liu, Hanqiao Fishbane, Nick Verleden, Stijn E. Vanaudenaerde, Bart M. Lenburg, Marc E. Stevenson, Christopher S. Spira, Avrum Cooper, Joel D. Hackett, Tillie-Louise Hogg, James C.
Description	Author Affiliation: Vasilescu DM ( University of British Columbia dragos.vasilescu@hli.ubc.ca.); Phillion AB ( McMaster University.); Tanabe N ( University of British Columbia.); Kinose D ( University of British Columbia.); Paige DF ( Paige Instruments.); Kantrowitz JJ ( Boston University Medical Center.); Liu G ( Boston University.); Liu H ( Boston University Medical Center.); Fishbane N ( University of British Columbia.); Verleden SE ( KU Leuven.); Vanaudenaerde BM ( KULeuven.); Lenburg ME ( Boston University.); Stevenson CS ( Janssen Pharmaceutical Companies of Johnson and Johnson.); Spira A ( Boston University School of Medicine.); Cooper JD ( University of Pennsylvania.); Hackett TL ( University of British Columbia.); Hogg JC ( University of British Columbia.)
Abstract	Micro Computed Tomography (CT) enables 3-dimensional (3D) imaging of complex soft tissue structures, but current protocols used to achieve this goal preclude cellular and molecular phenotyping of the tissue. Here we describe a radiolucent cryo stage that permits micro CT imaging of unfixed frozen human lung samples at a spatial resolution of 11 µm under conditions where the sample is maintained frozen at -30°C during imaging. The cryo stage was tested for thermal stability to maintain samples frozen up to 8 hours. This report describes the methods used to choose the materials required for cryo stage construction, demonstrates that whole genome mRNA integrity and expression is not compromised by exposure to micro CT radiation, and that the tissue can be used for immunohistochemistry. The new cryo stage provides a novel method enabling integration of 3D tissue structure with cellular and molecular analysis to facilitate the identification of molecular determinants of disease.
File Format	HTM / HTML
ISSN	87507587
e-ISSN	15221601
Journal	Journal of Applied Physiology
Language	English
Publisher	American Physiological Society
Publisher Date	2016-11-17
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Applied Physiology Molecular Biology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Sports Science

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