NDLI: Tug-of-war of microtubule filaments at the boundary of a kinesin- and dynein-patterned surface

Content Provider	PubMed Central
Author	Ikuta, Junya Kamisetty, Nagendra K. Shintaku, Hirofumi Kotera, Hidetoshi Kon, Takahide Yokokawa, Ryuji
Copyright Year	2014
Abstract	Intracellular cargo is transported by multiple motor proteins. Because of the force balance of motors with mixed polarities, cargo moves bidirectionally to achieve biological functions. Here, we propose a microtubule gliding assay for a tug-of-war study of kinesin and dynein. A boundary of the two motor groups is created by photolithographically patterning gold to selectively attach kinesin to the glass and dynein to the gold surface using a self-assembled monolayer. The relationship between the ratio of two antagonistic motor numbers and the velocity is derived from a force-velocity relationship for each motor to calculate the detachment force and motor backward velocity. Although the tug-of-war involves >100 motors, values are calculated for a single molecule and reflect the collective dynein and non-collective kinesin functions when they work as a team. This assay would be useful for detailed in vitro analysis of intracellular motility, e.g., mitosis, where a large number of motors with mixed polarities are involved.
Related Links	http://dx.doi.org/10.1038/srep05281
Starting Page	5281
File Format	PDF
ISSN	20452322
e-ISSN	20452322
Journal	Scientific Reports
Volume Number	4
Language	English
Publisher	Nature Publishing Group
Publisher Date	2014-06-01
Access Restriction	Open
Rights Holder	Nature Publishing Group
Subject Keyword	Science and technology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Tug-of-war of microtubule filaments at the boundary of a kinesin- and dynein-patterned surface

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