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Kinesin and dynein move a peroxisome in vivo: a tug-of-war or coordinated movement?
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kural, Comert Kim, Hwajin Syed, Sheyum Goshima, Gohta Gelfand, Vladimir I. Selvin, Paul R. |
| Copyright Year | 2005 |
| Abstract | We used fluorescence imaging with one nanometer accuracy (FIONA) to analyze organelle movement by conventional kinesin and cytoplasmic dynein in a cell. We located a green fluorescence protein (GFP)-tagged peroxisome in cultured Drosophila S2 cells to within 1.5 nanometers in 1.1 milliseconds, a 400-fold improvement in temporal resolution, sufficient to determine the average step size to be approximately 8 nanometers for both dynein and kinesin. Furthermore, we found that dynein and kinesin do not work against each other in vivo during peroxisome transport. Rather, multiple kinesins or multiple dyneins work together, producing up to 10 times the in vitro speed. |
| File Format | PDF HTM / HTML |
| DOI | 10.1126/science.1108408 |
| Alternate Webpage(s) | http://people.physics.illinois.edu/Selvin/PRS/PSCV/PTP/2005/Comert_Science_060305.pdf |
| Alternate Webpage(s) | http://people.physics.illinois.edu/Selvin/PRS/PSCV/PTP/2005/1108408Kural.Rev.pdf |
| Alternate Webpage(s) | http://fire.biol.wwu.edu/cmoyer/zztemp_fire/biol205_F05/papers/Kural_tugowar_sci05.pdf |
| PubMed reference number | 15817813 |
| Alternate Webpage(s) | https://doi.org/10.1126/science.1108408 |
| Journal | Medline |
| Volume Number | 308 |
| Issue Number | 5727 |
| Journal | Science |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
