NDLI: 8B-1 Polymer Contrast Particles For Cellular Imaging With Ultrasound and MRI

Content Provider	IEEE Xplore Digital Library
Author	Williamson, R. Aristizabal, O. Turnbull, D.H. Ketterling, J.A. Zaim, Y. Fahmy, T.M.
Copyright Year	2007
Description	Author affiliation: NYU, New York (Williamson, R.; Aristizabal, O.; Turnbull, D.H.)
Abstract	This study describes the fabrication and characterization of a targeted polymer-based contrast agent for multi-modal ultrasound and magnetic resonance imaging, providing the potential for cell labeling and tracking in vivo. Ultrasound and MRI contrast enhancing particles (UMCEP) were fabricated using a biodegradable polymer, poly(lactic-co-glycolic) acid (PLGA) particles encapsulating octafluoropropane and monocrystalline iron-oxide nanoparticles (MION). Targeting was achieved using an avidin-palmitate stabilizer which provided a high density of sites for conjugation of biotinylated ligands. Particle size analysis of the UMCEPs revealed a heterogeneous distribution of particles sizes with a mean diameter close to 1 mum (0.88 mumplusmn0.03, s.e.m) and scanning electron micrographs (SEM) showed a pronounced increase in surface roughness of avidin-coated UMCEPs compared to plain UMCEPs. UMCEPs caused a nine-fold increase in signal intensity compared to background at 40 MHz and higher harmonics (80 MHz and 120 MHZ peaks) were observed in the frequency spectra at multi-cycle, medium pressure excitation regimens. MRI phantom studies with UMCEPs showed T2-weighted signal darkening, with a measured relaxivity of 7.4 $s^{-1}$ at a concentration of 4 mg/ml of particles. Cell culture experiments with UMCEPs conjugated to human transferrin showed a two-fold increase in uptake of targeted UMCEPs by TfR expressing (TfR+) cells (3.46plusmn1.21 s.e.m particles/cell) compared to TfR negative (TfR-, control) (1.66plusmn0.32 s.e.m particles/cell).
Starting Page	648
Ending Page	651
File Size	2733720
Page Count	4
File Format	PDF
ISBN	9781424413836
ISSN	10510117
DOI	10.1109/ULTSYM.2007.168
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2007-10-28
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Ultrasonic imaging Polymers Magnetic resonance imaging Fabrication Labeling Target tracking In vivo Biodegradable materials Nanoparticles Electrons
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in