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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Park, Mi Kyoung Arya, Sunil K. Kongsuphol, Patthara |
| Description | Author Affiliation: Arya SK ( Institute of Microelectronics, Agency for Science Technology and Research (A*STAR), 2 Fusionopolis Way, #08-02 Innovis Tower, Singapore 138634, Singapore. Electronic address: sunilarya333@gmail.com.); Kongsuphol P ( Institute of Microelectronics, Agency for Science Technology and Research (A*STAR), 2 Fusionopolis Way, #08-02 Innovis Tower, Singapore 138634, Singapore.); Park MK ( Institute of Microelectronics, Agency for Science Technology and Research (A*STAR), 2 Fusionopolis Way, #08-02 Innovis Tower, Singapore 138634, Singapore.) |
| Abstract | The manuscript presents a new biosensor platform using bioreceptors modified porous 2-dimensional (2D) membrane based off-surface matrix for on-chip electrochemical immunoassay. Antibody based bioreceptors modified 2D matrix of porous polycarbonate (PC) membrane with densely packed 20µm holes as off-surface matrix was incorporated in very close proximity of the sensor surface and integrated with fluidic system for reagent flow and incubation chamber. Covalent attachment of antibodies on 2D PC membrane based off-matrix was achieved using 4-fluoro-3-nitro-azidobenzene (FNAB) cross-linker. Anti-TNF- /FNAB/PC membrane was integrated over array of micro fingers of gold based sensor chip using double side tape spacer and StartingBlock phosphate buffer saline- Tween-20; (PBS-T20) blocking buffer was utilized to minimize nonspecific binding. Differential pulse voltammetric studies of Anti-Tnf- /FNAB/PC-Au for protein biomarker (TNF- ) detection and estimation in undiluted serum indicated that the immunosensor system can detect TNF- linearly in 100pg/ml to 100ng/ml range with insignificant interference from other cytokines and serum proteins. Further, immunosensor exhibited high sensitivity of 194nA/(ng/ml) and 240nA/(ng/ml), respectively for single and double membrane based system. Thus, use of 2D membrane based off surface matrix may present the new platform to sensitively measure biomarkers electrochemically to pg/ml range with insignificant nonspecific binding and false signal in undiluted serum. |
| ISSN | 09565663 |
| Volume Number | 91 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2017-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Antibodies, Immobilized Chemistry Immunoassay Instrumentation Lab-on-a-chip Devices Membranes, Artificial Tumor Necrosis Factor-alpha Blood Azides Biological Markers Biosensing Techniques Cross-linking Reagents Electrochemical Techniques Equipment Design Gold Humans Limit Of Detection Nitrobenzenes Polycarboxylate Cement Evaluation Studies Journal Article Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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