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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Yu, Zhigang He, Xunjun Yu, Xiang Li, Fengqin Shi, Wenbing Xu, Yanmei Zhang, Guiling Yan, Hong Xu, Lan |
| Description | Country affiliation: China Author Affiliation: Yu X ( College of Chemical Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China.); Yu Z ( Department of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling, Chongqing 408100, China); Li F ( College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China.); Xu Y ( College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China.); He X ( Department of Electronic Science and Technology, Harbin University of Science and Technology, Harbin 150080, China.); Xu L ( Key Laboratory on Luminescence and Real-time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.); Shi W ( Department of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling, Chongqing 408100, China.); Zhang G ( College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China.); Yan H ( College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China.) |
| Abstract | A type of 'signal on' displacement-based sensors named target induced signaling probe shifting DNA-based (TISPS-DNA) sensor were developed for a designated DNA detection. The signaling mechanism of the signaling probe (SP) shifting different from the classical conformation/flexibility change mode endows the sensor with high sensitivity. Through using thiolated or no thiolated capturing probe (CP), two 3-probe sensing structures, sensor-1 and sensor-2, were designed and constructed. The systematical comparing research results show that both sensors exhibit some similarities or big differences in sensing performance. On the one hand, the similarity in structures determines the similarity in some aspects of signaling mechanism, background signal, signal changing form, anti-fouling ability and versatility; on the other hand, the slight difference in structures also results in two opposite hybridization modes of gradual increasing resistance and gradual decreasing resistance which can affect the hybridization efficiency between the assistant probe (AP) and the SP, further producing some big differences in sensing performance, for example, apparently different signal enhancement (SE) change, point mutation discrimination ability and response speed. Under the optimized fabrication and detection conditions, both sensors feature high sensitivity for target DNAs with the detection limits of â ¼10 fM for sensor-1 and â ¼7 fM for sensor-2, respectively. Among many acquired sensing virtues, the sensor-1 shows a peculiar specificity adjustability which is also a highlight in this work. |
| ISSN | 09565663 |
| Volume Number | 91 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2017-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Methods Dna Probes Chemistry Dna Analysis Nucleic Acid Hybridization Genetics Electrochemical Techniques Electrodes Nucleic Acid Conformation Point Mutation Journal Article Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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