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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Han, De-Man Shi, Wei Zhang, Siqi Chen, Xiaoying Jia, Wen-Ping Sun, Ting Wang, Kun Li, Kai-Bin |
| Description | Author Affiliation: Zhang S ( Department of Chemistry, Taizhou University, Jiaojiang 318000, China); Wang K ( College of Sciences, Northeastern University, Shenyang 110819, China.); Li KB ( Department of Chemistry, Taizhou University, Jiaojiang 318000, China.); Shi W ( Department of Chemistry, Taizhou University, Jiaojiang 318000, China.); Jia WP ( Department of Chemistry, Taizhou University, Jiaojiang 318000, China.); Chen X ( Department of Chemistry, Taizhou University, Jiaojiang 318000, China.); Sun T ( College of Sciences, Northeastern University, Shenyang 110819, China.); Han DM ( Department of Chemistry, Taizhou University, Jiaojiang 318000, China. Electronic address: hdm@tzc.edu.cn.) |
| Abstract | A silver nanoclusters (AgNCs)/graphene oxide (GO)-based fluorescence sensor was developed for label-free DNA detection through hybridization chain reaction (HCR). A DNA sequence associated with the human immunodeficiency virus (HIV) was selected as a model target. Two DNA probes, hairpin probe 1 (H ) and hairpin probe 2 (H ), were partially complementary. GO was used as an adsorption material to capture the hairpin probes and a selective fluorescence quencher was used to reduce the background signal. Upon addition of AgNO and NaBH , the AgNCs were synthesized at the terminals of the H and H probes. In the absence of target DNA (T ), hybridization chain reaction (HCR) could not be triggered due to the stability of H and H probes. The hairpin probe-protected AgNCs attached to the GO surface, efficiently quenching fluorescence of the AgNCs. Therefore, the system showed very low background. In presence of T , the target triggered the chain-like assembly of H and H through HCR, generating a long chain of H and H complexes. The HCR product (AgNCs nanowires) could not be adsorbed on the surface of GO; hence, it generated a strong fluorescent signal based on the concentration of the target. Under the optimized conditions, the detection limit of the fluorescence sensor was 1.18nM, and hence it can be applied to clinical samples. |
| ISSN | 09565663 |
| Journal | Biosensors and Bioelectronics |
| Volume Number | 91 |
| e-ISSN | 18734235 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2017-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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