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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Chen, Xiaojun Yao, Cheng Zhang, Qi Tu, Fulai |
| Description | Author Affiliation: Zhang Q ( College of Sciences, Nanjing Tech University, Nanjing 211816, PR China.); Chen X ( College of Sciences, Nanjing Tech University, Nanjing 211816, PR China. Electronic address: chenxj_njut@126.com.); Tu F ( Nanjing Sichuang Biotechnology Co. Ltd., Nanjing 211800, PR China.); Yao C ( College of Sciences, Nanjing Tech University, Nanjing 211816, PR China. Electronic address: yaocheng@njtech.edu.cn.) |
| Abstract | The measurement of free thyroxine concentration in serum is considered to be an essential indicator of thyroid function. Here, a novel enzyme-free sandwich electrochemical immunosensor for the detection of FT4 antigen based on the immobilization of primary antibody (Ab1) on three dimensional ordered macroporous chitosan-Au nanoparticles hybrid (3DOM CS-AuNPs) film electrode, and magnetic multiwall carbon nanotubes (MMWCNTs) were used as label of secondary antibody (Ab2). The 3DOM CS-AuNPs film electrode was constructed by one-step electrodeposition of CS-AuNPs composite onto Au electrode with silica opal template. MMWCNTs were prepared by chemical co-precipitation of Fe(2+) and Fe(3+) salts on carboxylated MWCNTs. Ru(bpy)3(2+) labeled anti-FT4 (Ru(bpy)3(2+)-Ab2) was covalently attached to MMWCNTs through the formation of amide bond between the carboxylic groups of MWCNTs and the amine groups of antibody. Under the optimal conditions, FT4 was detected in a concentration range from 0.71 fg mL(-1) to 1.15 pg mL(-1) with a correlation coefficient of 0.998 and a detection limit of 0.20 fg mL(-1). Moreover, the immunosensor showed excellent selectivity, good stability, satisfactory reproducibility and regeneration. Importantly, the developed method was used to assay clinical serum specimens, achieving a good relation with those obtained from the commercialized electrochemiluminescent method. |
| ISSN | 09565663 |
| Volume Number | 59 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-09-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Chitosan Chemistry Electrochemical Techniques Instrumentation Gold Nanoparticles Thyroxine Blood Antibodies, Immobilized Biosensing Techniques Equipment Design Humans Immunoassay Limit Of Detection Ultrastructure Nanotubes, Carbon Reproducibility Of Results Analysis Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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