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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Pandey, Ashish Qureshi, Anjum Gurbuz, Yasar Ozguz, Volkan Niazi, Javed H. |
| Description | Country affiliation: Turkey Author Affiliation: Pandey A ( Sabanci University Nanotechnology Research and Application Center, Orta Mah., 34956 Istanbul, Turkey.); Gurbuz Y ( Faculty of Engineering and Natural Science, Sabanci University, Orhanli, Tuzla, 34956 Istanbul, Turkey.); Ozguz V ( Sabanci University Nanotechnology Research and Application Center, Orta Mah., 34956 Istanbul, Turkey.); Niazi JH ( Sabanci University Nanotechnology Research and Application Center, Orta Mah., 34956 Istanbul, Turkey. Electronic address: javed@sabanciuniv.edu.); Qureshi A ( Sabanci University Nanotechnology Research and Application Center, Orta Mah., 34956 Istanbul, Turkey. Electronic address: anjum@sabanciuniv.edu.) |
| Abstract | E. coli O157:H7 is an enterohemorrhagic bacteria responsible for serious foodborne outbreaks that causes diarrhoea, fever and vomiting in humans. Recent foodborne E. coli outbreaks has left a serious concern to public health. Therefore, there is an increasing demand for a simple, rapid and sensitive method for pathogen detection in contaminated foods. In this study, we developed a label-free electrical biosensor interfaced with graphene for sensitive detection of pathogenic bacteria. This biosensor was fabricated by interfacing graphene with interdigitated microelectrodes of capacitors that were biofunctionalized with E. coli O157:H7 specific antibodies for sensitive pathogenic bacteria detection. Here, graphene nanostructures on the sensor surface provided superior chemical properties such as high carrier mobility and biocompatibility with antibodies and bacteria. The sensors transduced the signal based on changes in dielectric properties (capacitance) through (i) polarization of captured cell-surface charges, (ii) cells' internal bioactivity, (iii) cell-wall's electronegativity or dipole moment and their relaxation and (iv) charge carrier mobility of graphene that modulated the electrical properties once the pathogenic E. coli O157:H7 captured on the sensor surface. Sensitive capacitance changes thus observed with graphene based capacitors were specific to E. coli O157:H7 strain with a sensitivity as low as 10-100 cells/ml. The proposed graphene based electrical biosensor provided advantages of speed, sensitivity, specificity and in-situ bacterial detection with no chemical mediators, represents a versatile approach for detection of a wide variety of other pathogens. |
| ISSN | 09565663 |
| Journal | Biosensors and Bioelectronics |
| Volume Number | 91 |
| e-ISSN | 18734235 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2017-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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