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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Dulay, Samuel B. O'Sullivan, Ciara K. Julich, Sandra Tomaso, Herbert Gransee, Rainer |
| Description | Author Affiliation: Dulay SB ( Nanobiotechnology and Bioanalysis Group, INTERFIBIO, Departament d׳Enginyeria Quimica, Universitat Rovira i Virgili, Avinguda Països Catalans 26, 43007 Tarragona, Spain. Electronic address: samuelbacena.dulay@estudiants.urv.cat.); Gransee R ( Fraunhofer ICT-IMM, Carl-Zeiss-Strasse 18-20, 55129 Mainz, Germany.); Julich S ( Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, Naumburger Strasse 96 a, Jena 07743, Germany.); Tomaso H ( Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, Naumburger Strasse 96 a, Jena 07743, Germany.); O'Sullivan CK ( Nanobiotechnology and Bioanalysis Group, INTERFIBIO, Departament d׳Enginyeria Quimica, Universitat Rovira i Virgili, Avinguda Països Catalans 26, 43007 Tarragona, Spain) |
| Abstract | Tularemia is a highly infectious zoonotic disease caused by a Gram-negative coccoid rod bacterium, Francisella tularensis. Tularemia is considered as a life-threatening potential biological warfare agent due to its high virulence, transmission, mortality and simplicity of cultivation. In the work reported here, different electrochemical immunosensor formats for the detection of whole F. tularensis bacteria were developed and their performance compared. An anti-Francisella antibody (FB11) was used for the detection that recognises the lipopolysaccharide found in the outer membrane of the bacteria. In the first approach, gold-supported self-assembled monolayers of a carboxyl terminated bipodal alkanethiol were used to covalently cross-link with the FB11 antibody. In an alternative second approach F(ab) fragments of the FB11 antibody were generated and directly chemisorbed onto the gold electrode surface. The second approach resulted in an increased capture efficiency and higher sensitivity. Detection limits of 4.5 ng/mL for the lipopolysaccharide antigen and 31 bacteria/mL for the F. tularensis bacteria were achieved. Having demonstrated the functionality of the immunosensor, an electrode array was functionalised with the antibody fragment and integrated with microfluidics and housed in a tester set-up that facilitated complete automation of the assay. The only end-user intervention is sample addition, requiring less than one-minute hands-on time. The use of the automated microfluidic set-up not only required much lower reagent volumes but also the required incubation time was considerably reduced and a notable increase of 3-fold in assay sensitivity was achieved with a total assay time from sample addition to read-out of less than 20 min. |
| ISSN | 09565663 |
| Volume Number | 59 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-09-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Francisella Tularensis Isolation & Purification Lipopolysaccharides Analysis Microfluidic Analytical Techniques Tularemia Diagnosis Antibodies, Immobilized Chemistry Electrochemical Techniques Equipment Design Humans Immunoassay Lab-on-a-chip Devices Models, Molecular Microbiology Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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